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Kit for carrying out fluorescence quantitative on ractopamine through and preparation method of fluorescence labeling liquid

A fluorescent quantitative detection and ractopamine technology, which is applied in the field of medical testing, can solve problems affecting the sensitivity and accuracy of detection results, complex production processes, and difficult quality control of detection limits, achieving easy operation, good accuracy, and responsiveness. Uniform effect with signal release

Inactive Publication Date: 2012-01-18
GUANGZHOU WONDFO BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the detection by the colloidal gold method has the disadvantages of poor sensitivity and difficult quality control of the detection limit.
In addition, the disadvantages of using marker pads for solid-phase reactions are: the production process is complicated, and the results of each reaction have large discrepancies, and the errors are large, which affects the sensitivity and accuracy of the detection results.

Method used

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  • Kit for carrying out fluorescence quantitative on ractopamine through and preparation method of fluorescence labeling liquid
  • Kit for carrying out fluorescence quantitative on ractopamine through and preparation method of fluorescence labeling liquid
  • Kit for carrying out fluorescence quantitative on ractopamine through and preparation method of fluorescence labeling liquid

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] In this embodiment, the immunochromatographic kit for fluorescent quantitative detection of ractopamine includes a test strip and a fluorescent labeling solution.

[0034] Wherein, according to the conventional method of the test strip, the test strip is overlapped successively by the sample pad 1, the nitrocellulose coating film 2 including the detection area (T area) 3 and the quality control area (C area) 4, and the absorbent paper 5 Pasted on the bottom plate 6 and constituted, such as figure 1 and figure 2 shown.

[0035] In this embodiment, 0.5 mg / ml RCT-BSA conjugate (ie, ractopamine antigen) coating solution was used at the T line of the detection area of ​​the coating membrane, and the usage volume was 90 ul / 27 cm. Anti-rabbit IgG with a concentration of 1mg / ml was used to coat the C line of the quality control area of ​​the coated membrane, and the amount used was the same as 90ul / 27cm. Used to bind fluorescently labeled rabbit IgG for testing the validity...

Embodiment 2

[0040] In this embodiment, the immunochromatographic kit for fluorescent quantitative detection of ractopamine includes a test strip and a fluorescent labeling solution.

[0041] Wherein, the test strip consists of a sample pad, a cellulose-coated film including a detection area (T area) and a quality control area (C area), and absorbent paper, which are successively pasted on the base plate by overlapping each other according to the conventional method of the test paper.

[0042] In this embodiment, 1 mg / ml RCT-BSA conjugate (ie, ractopamine antigen) coating solution was used at the T line of the detection area of ​​the coated membrane, and the amount used was 90 ul / 35 cm. Use anti-rabbit IgG with a concentration of 1mg / ml on the C line of the quality control area of ​​the coating membrane, and the amount used is the same as 90ul / 35cm, which is used to bind fluorescently-labeled rabbit IgG to detect the effectiveness of the test strip. .

[0043] In this embodiment, after th...

Embodiment 3

[0047] In this embodiment, the immunochromatographic kit for fluorescent quantitative detection of ractopamine includes a test strip and a fluorescent labeling solution.

[0048] Wherein, the test strip consists of a sample pad, a cellulose-coated film including a detection area (T area) and a quality control area (C area), and absorbent paper, which are successively pasted on the base plate by overlapping each other according to the conventional method of the test paper.

[0049] In this embodiment, 1.5 mg / ml RCT-BSA conjugate (ie, ractopamine antigen) coating solution was used at the T line of the detection area of ​​the coated membrane, and the usage volume was 90 ul / 35 cm. Use anti-rabbit IgG with a concentration of 2mg / ml on the C line of the quality control area of ​​the coating membrane, and the amount used is the same as 90ul / 35cm, which is used to bind fluorescently-labeled rabbit IgG to detect the effectiveness of the test strip. .

[0050] In this embodiment, the f...

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Abstract

The invention discloses an immunochromatographic kit for carrying out fluorescence quantitative on ractopamine through and a preparation method of a fluorescence labeling liquid. The kit comprises a test strip and the fluorescence labeling liquid, wherein the test strip is formed by sequentially overlapping and adhering a sample pad, a nitrocellulose coating film, and absorbent paper on a bottom plate; the nitrocellulose coating film comprises a detection region and a quality control region; the detection region is coated with an RCT-BSA (antibody against Ractopamine-bovine serum albumin) conjugate, the quality control region is coated with anti-rabbit IgG; and the fluorescence labeling liquid contains a fluorescence labeling RCT antibody and a fluorescence labeling rabbit IgG. Compared with the immune colloidal gold labeling test strip, the kit has the advantages of higher sensitivity, accurate quantization and the like, and is more convenient and rapidly in operation in comparison with an enzyme coupling method.

Description

technical field [0001] The invention belongs to the field of medical testing, in particular to an immunochromatographic detection kit for fluorescent quantitative detection of ractopamine (RCT) and a preparation method of a fluorescent labeling solution. Background technique [0002] Common stimulants include clenbuterol, ractopamine, albuterol, etc. Clenbuterol is commonly known as "clenbuterol". With increased regulation of clenbuterol in China, the use of ractopamine has gradually decreased and the use of other beta-agonists has gradually increased. Ractopamine is a newly developed β-stimulant in the United States. In December 1999, the U.S. Food and Drug Administration (FDA) approved ractopamine to be used in pig breeding. Ractopamine is also the only β-stimulant allowed in the European Union. There are also research patent reports in China, and ractopamine is being widely used as a substitute for clenbuterol. However, the Chinese Ministry of Agriculture, the Ministry ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/558G01N33/52G01N33/533G01N21/64
Inventor 王继华刘晓云唐海波
Owner GUANGZHOU WONDFO BIOTECH
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