Method for diagnosing Nigrospora oryzae (Berk. et Br.) Petch. of seed corn and identifying pathogeny

A technology of seed production corn and identification method, which is applied in the direction of microbe-based method, biochemical equipment and method, microbe measurement/inspection, etc., can solve the problems of symptom manifestation, pathogenic bacteria identification, etc., which are still blank in China, and achieve diagnosis The method is simple, easy to operate, easy to obtain, and the effect of the diagnostic method is accurate

Active Publication Date: 2012-01-18
甘肃省敦煌种业股份有限公司玉门市种子公司
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Problems solved by technology

Maize split shaft disease [Nigrospora oryzae (Berk.et Br.) Petch.] is a new disease found in inbred and hybrid corn in Gansu Province. The research on identification of pathogenic bacteria is still blank in China

Method used

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Embodiment Construction

[0025] The method for diagnosing and identifying the pathogen of corn split shaft disease in seed production comprises the following steps:

[0026] (1) Symptom diagnosis of corn split shaft disease in seed production: observe the symptoms and symptoms of the leaves, ears and seeds at the seedling stage of the seed production corn field-jointing stage, tasseling stage, ear stage, harvest stage, and storage stage, determination--

[0027] When the tips of corn bracts are dead, the bracts are yellow and dead, there is black powder on the ear and the inner side of the bract close to the ear, and there are white filaments on the kernels of the ear or between the rows of kernels—that is, the mycelium of the pathogen, That is, it is determined that the ear is moldy.

[0028] When the cobs from which the corn kernels have been removed become soft, black moldy matter appears on the inner surface of the cobs after the cobs are cut open, and when microscopically detected as pathogenic ...

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Abstract

The invention relates to a method for diagnosing Nigrospora oryzae (Berk. et Br.) Petch. of seed corn and identifying pathogeny. The method comprises the following steps of: (1) diagnosing symptoms of the Nigrospora oryzae (Berk. et Br.) Petch. of the seed corn, namely observing part symptom representation and disease sign representation of leaves, clusters and seeds from a seedling stage to a jointing stage, a tasseling stage, a spike stage, a harvesting stage and a storage stage of the seed corn respectively, and judging; and (2) identifying the pathogeny of the Nigrospora oryzae (Berk. et Br.) Petch., namely judging fresh diseased leaves and diseased grains which are diagnosed in step (1) in a way of moisturized induction and separation. The method is simple, quick, accurate and easy to operate; and the phenomenon that the misdiagnosis is caused by diagnosing diseases according to the characteristics showed in one growth period is avoided.

Description

technical field [0001] The invention relates to a disease diagnosis technology and pathogen identification of corn for seed production, in particular to a method for diagnosis and pathogen identification of corn crack disease in seed production. Background technique [0002] Gansu Province has become a major province of maize seed production in the country due to its unique natural conditions. With the extensive introduction of maize germplasm resources, new inbred lines and hybrids are constantly coming out, and new diseases that endanger maize production are constantly emerging. The occurrence of diseases has become the key to restricting the production of corn, resulting in reduced yield and quality. Therefore, according to the regional characteristics, it is particularly important to strengthen the research on new corn diseases. [0003] In recent years, a new disease of corn seed production in the Hexi Corridor—maize split shaft disease has become a serious constraint ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/04C12R1/645
Inventor 闫治斌雷玉明
Owner 甘肃省敦煌种业股份有限公司玉门市种子公司
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