Dynamic Surface Enhanced Raman Spectroscopy Detection Method

Abstract

The invention belongs to an analysis and detection technology, in particular to a surface-enhanced Raman spectrum detection method, in which ethanol is added dropwise to a SERS sink bottom containing a target molecule to be measured, and then the signal of the target is detected under a Raman spectrometer. The detection method of the invention can increase the detection limit of the traditional SERS substrate by 104-1010 orders of magnitude, so that Raman spectroscopy can be deeply applied in the field of trace and ultra-trace detection.

Description

[0001] technical field [0002] The invention belongs to an analysis and detection technology, in particular to a surface-enhanced Raman spectrum detection method. Background technique [0003] Raman spectroscopy, like infrared spectroscopy, belongs to molecular vibration spectroscopy, which can reflect the characteristic structure of molecules. However, the Raman scattering effect is a very weak process, and generally its light intensity is only about 10 of the incident light intensity. -10 . Therefore, the Raman signals are very weak, and some kind of enhancement effect is almost always used to study the Raman spectroscopy of surface adsorbed species. When the selected incident laser wavelength is very close to or within the range of electronic absorption peaks of scattering molecules, the probability of Raman transition is greatly increased, making the Raman scattering cross section of some vibrational modes of molecules enhanced up to 10 6 times, this phenomenon ...

AI Technical Summary

Problems solved by technology

For more than three decades, despite detection limits as high as 10 -12 -10 -14 M's SERS substrates have been reported, but their preparation techniques and operating conditions are extremely complicated, and the operation is cumbersome, and they can only stay in the laboratory stage, so it is difficult to promote their use.

Too complicated SERS substrate preparation and programming scheme has no practical and commercial value in terms of detection cost

In addition, the research energy consumption of these ultra-trace SERS substrates is very high, which is not advisable from the perspective of low carbon and environmental protection

However, when most commercialized SERS substrates adopt the traditional SERS detection scheme, the detection limit is difficult to exceed 10 -6 M, so there will be no advantage in the field of trace analysis and detection applications

The traditional SERS technology detection scheme is difficult to be applied in field detection, and its research results can only stay in the laboratory stage

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