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Oxidation resisting polypeptide and preparation method thereof

An antioxidant polypeptide and antioxidant activity technology, applied in the biological field, can solve problems such as unfavorable food preservation, and achieve the effects of improving the low utilization rate of collagen, solving the problem of reuse, and alleviating food safety concerns.

Active Publication Date: 2011-10-19
FUZHOU UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

α-tocopherol is the most commonly used natural antioxidant, it can effectively maintain the stability of oil in food, but it is not conducive to food preservation

Method used

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  • Oxidation resisting polypeptide and preparation method thereof
  • Oxidation resisting polypeptide and preparation method thereof
  • Oxidation resisting polypeptide and preparation method thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0049] Weigh 7.5 grams of shark skin collagen, dissolve it in deionized water and make it up to 250ml, then adjust its pH to 3.0 with 2mol / L HCl. First heat the solution to 50°C in a water bath, then add a corresponding amount of enzyme according to the enzyme-substrate ratio of 3000 U / g, and the enzymatic hydrolysis time is 5 hours. Then inactivate the enzyme in a boiling water bath for 15 minutes, then centrifuge at 4000 rpm for 15 minutes after cooling. Collect the supernatant for later use.

[0050] The supernatant was separated by ultrafiltration with an ultrafiltration membrane with a molecular weight cut-off range of 8000Da and 3500Da, and it was divided into three components with different molecular weight ranges, namely components with a molecular weight greater than 8000Da and components with a molecular weight between 3500Da and 8000Da. Components, components with a molecular weight less than 3500Da, and their antioxidant activity (such as figure 1 shown). It can...

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Abstract

The invention provides an oxidation resisting polypeptide and a preparation method thereof. The method takes sharkskin collagen as the raw material. By means of enzymolysis of acidic protease, separation and purification, a specific oxidation resisting polypeptide can be obtained, with a molecular weight of 902Da and a complete amino acid sequence as Gly-Thr-Met-Gly-Ala-Val-Gly-Pro-Arg-Gly. The oxidation resisting polypeptide of the invention eliminates the defects of natural antioxidants and relieves the public from their anxiety about synthetic antioxidant, thus establishing the theoretical foundation for developing oxidation resisting polypeptide based on food sources and exploring the wide application of the oxidation resisting polypeptide in food and medical science.

Description

technical field [0001] The invention provides an antioxidant polypeptide and a preparation method thereof, belonging to the field of biotechnology. Background technique [0002] Oxidation of biomolecules is a free radical-mediated process that can have many adverse effects on food and biological systems. In aerobic organs, free radicals related to many diseases such as arteriosclerosis and cancer will inevitably be produced along with the process of oxygen metabolism. In food, the oxidation of food nutrients will produce peroxides, which will not only affect the nutritional value of food, cause food quality to decline, and even cause diseases in the body of the ingestor. Therefore, finding safe antioxidants to inhibit the production of peroxides has always been a research hotspot in biochemical nutrition. Because chemically synthesized antioxidants such as BHT and TBHQ have better effects and cheaper prices than natural antioxidants, they have been widely used in the food ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K7/06C07K1/36C07K1/34C07K1/20C07K1/18C07K1/16C12P21/06
Inventor 汪少芸江勇林月萍王军祝贝贝赵立娜赵珺
Owner FUZHOU UNIV
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