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Antioxidant polypeptide prepared by enzymatic hydrolysis of sharkskin collagen with acid protease

An antioxidant polypeptide and acid protease technology, applied in the biological field, can solve problems such as unfavorable food preservation, and achieve the effects of improving the low utilization rate of collagen, solving the problem of reuse, and alleviating food safety concerns.

Active Publication Date: 2011-10-19
FUZHOU UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

α-tocopherol is the most commonly used natural antioxidant, it can effectively maintain the stability of oil in food, but it is not conducive to food preservation

Method used

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  • Antioxidant polypeptide prepared by enzymatic hydrolysis of sharkskin collagen with acid protease
  • Antioxidant polypeptide prepared by enzymatic hydrolysis of sharkskin collagen with acid protease
  • Antioxidant polypeptide prepared by enzymatic hydrolysis of sharkskin collagen with acid protease

Examples

Experimental program
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Effect test

Embodiment 1

[0049] Weigh 7.5 grams of shark skin collagen, dissolve it in deionized water and make it up to 250ml, then adjust its pH to 3.0 with 2mol / L HCl. First heat the solution to 50°C in a water bath, then add a corresponding amount of enzyme according to the enzyme-substrate ratio of 3000 U / g, and the enzymatic hydrolysis time is 5 hours. Then inactivate the enzyme in a boiling water bath for 15 minutes, then centrifuge at 4000 rpm for 15 minutes after cooling. Collect the supernatant for later use.

[0050] The supernatant was separated by ultrafiltration with an ultrafiltration membrane with a molecular weight cut-off range of 8000Da and 3500Da, and it was divided into three components with different molecular weight ranges, namely components with a molecular weight greater than 8000Da and components with a molecular weight between 3500Da and 8000Da. Components, components with a molecular weight less than 3500Da, and their antioxidant activity (such as figure 1 shown). It can...

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Abstract

The invention provides an antioxidant polypeptide prepared by enzymatic hydrolysis of sharkskin collagen with acid protease. According to the invention, sharkskin collagen is used as the raw material; after enzymatic hydrolysis with acid protease, the sharkskin collagen is subjected to isolation and purification so as to obtain a specific antioxidant polypeptide, the complete amino acid sequence of which is as follows: Gly-Pro-Ala-Gly-Pro-Ala-Gly-Ala-Ala-Gly. The invention enables the elimination of shortcomings of natural anti-oxidants and of public worry about synthetic anti-oxidants and lays a theoretical basis for the development of food derived antioxidant polypeptides and for the exploration of their application in foodstuff and medical science.

Description

technical field [0001] The invention provides an antioxidant polypeptide and a preparation method thereof, and more specifically relates to an antioxidant polypeptide prepared by hydrolyzing shark skin collagen with acid protease, which belongs to the field of biotechnology. Background technique [0002] Oxidation of biomolecules is a free radical-mediated process that can have many adverse effects on food and biological systems. In aerobic organs, free radicals related to many diseases such as arteriosclerosis and cancer will inevitably be produced along with the process of oxygen metabolism. In food, the oxidation of food nutrients will produce peroxides, which will not only affect the nutritional value of food, cause food quality to decline, and even cause diseases in the body of the ingestor. Therefore, finding safe antioxidants to inhibit the production of peroxides has always been a research hotspot in biochemical nutrition. Because chemically synthesized antioxidant...

Claims

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Application Information

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IPC IPC(8): C07K7/06C07K1/36C07K1/34C07K1/20C07K1/18C07K1/16C12P21/06
Inventor 汪少芸江勇赵立娜祝贝贝王军林月萍赵珺
Owner FUZHOU UNIV
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