Antioxidation polypeptide prepared from sharkskin collagen
An antioxidant peptide and collagen technology, applied in the biological field, can solve the problems of unfavorable food preservation, etc., achieve the effect of improving the low utilization rate of collagen, solving the problem of reuse, and eliminating side effects
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[0048] Weigh 7.5 grams of shark skin collagen, dissolve it in deionized water and make it up to 250ml, then adjust its pH to 3.0 with 2mol / L HCl. First heat the solution to 50°C in a water bath, then add a corresponding amount of enzyme according to the enzyme-substrate ratio of 3000 U / g, and the enzymatic hydrolysis time is 5 hours. Then inactivate the enzyme in a boiling water bath for 15 minutes, then centrifuge at 4000 rpm for 15 minutes after cooling. Collect the supernatant for later use.
[0049] The supernatant was separated by ultrafiltration with an ultrafiltration membrane with a molecular weight cut-off range of 8000Da and 3500Da, and it was divided into three components with different molecular weight ranges, namely components with a molecular weight greater than 8000Da and components with a molecular weight between 3500Da and 8000Da. Components, components with a molecular weight less than 3500Da, and their antioxidant activity (such as figure 1 shown). It can...
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