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Method for detecting safety of insect-resistant transgenic corn on non-target organisms

An insect-resistant gene and safe technology, which is applied in the field of detecting whether transgenic insect-resistant corn is safe to non-target organisms, can solve the problems of impossible non-target biological safety, and achieve convenient, fast and accurate investigation and counting. The effect of judgment

Inactive Publication Date: 2011-08-17
CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] For safety testing of genetically modified organisms, currently only some dominant species or economically important species such as silkworms can be selected, and it is impossible to use experiments to prove the safety of genetically modified crops to all non-target organisms

Method used

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  • Method for detecting safety of insect-resistant transgenic corn on non-target organisms
  • Method for detecting safety of insect-resistant transgenic corn on non-target organisms
  • Method for detecting safety of insect-resistant transgenic corn on non-target organisms

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0063] Embodiment 1, the detection of whether transgenic Bt corn is safe to non-target organisms

[0064] In 2009 and 2010, leafhoppers were used to study the effects of Cry1Ac transgenic maize on non-target organisms at the transgenic crop test base of China Agricultural University in Beijing.

[0065] 1. Method

[0066] 1. Material selection

[0067] 2009: (1) transgenic maize group (denoted as transgenic); (2) parental control group (denoted as parent).

[0068] 2010: (1) Transgenic corn group (denoted as transgenic); (2) parental control group (denoted as parent); (3) conventional hybrid varieties (denoted as Xianyu).

[0069] 2. Location selection

[0070] The test site is more than 500 meters away from other corn fields, and the fertility conditions of the test plots are basically the same. Each plot is about 25×30 meters, divided into 15 plots, and each variety has 5 replicates. The area of ​​each plot is set at 45m 2 (5m×9m), 15 rows are planted in each plot, 20 ...

Embodiment 2

[0157] Embodiment 2, the influence of transgenic corn on the growth and development of Heterochromia

[0158] Toxicology experiment: every 30 newly hatched ladybug larvae is a group, a total of 8 groups. Among them, 4 groups were fed with transgenic Bt corn pollen and aphids at a ratio of 2:1 (weight ratio) as treatment; 4 groups were fed with non-transgenic corn pollen and aphids at a ratio of 2:1 as a control. Ladybug larvae were single-headed in finger tubes, and their growth and development were observed every day, and the developmental periods of ladybugs at different ages were recorded. They were weighed every other day from larvae to early adult stages, and were weighed at the adult stage (45 days, Counting from the 1st instar) for the last weighing to evaluate the impact of transgenic insect-resistant corn pollen on ladybugs.

[0159] The result is as Figure 9 shown. The results showed that the GM corn was not toxic to Heterochromia.

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Abstract

The invention discloses a method for detecting safety of insect-resistant transgenic corn on non-target organisms. The method for detecting the safety of the insect-resistant transgenic corn on the non-target organisms comprises the following steps of: selecting insects with sucking mouth parts in the non-target organisms as a population to be detected, detecting whether an insect-resistant transgenic plant is safe for the population to be detected, if so, determining that the insect-resistant transgenic plant is safe for the non-target organisms in candidate, otherwise, and determining that the insect-resistant transgenic plant is not safe for the non-target organisms in candidate. The method makes up the defect that the insects with chewing mouth parts are separately used as research objects in the field of influence research of the transgenic corn on the non-target organisms, has significance for completing the influence research of transgenic crops on the non-target organisms, and has significance for determining the biological safety of the transgenic plants.

Description

technical field [0001] The invention relates to a method for detecting whether the transgenic insect-resistant gene corn is safe to non-target organisms. Background technique [0002] The main problem with traditional chemical insecticides is that they are toxic to other insects, animals and even humans who are not harmful. While killing target pests, pesticides also kill non-target insects, such as secondary pests, neutral insects, important economic insects, natural enemies of pests, and other non-target organisms, such as soil microorganisms, thereby disrupting the ecological balance. The application of insect-resistant transgenic crops is an important way to replace insecticides. [0003] Insect-resistant transgenic crops may also have potential negative effects, such as pest resistance to insect-resistant genes and impact on non-target organisms. It is of great significance for the healthy development of transgenic technology to conduct in-depth research on the potent...

Claims

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Application Information

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IPC IPC(8): G01N33/48
Inventor 石旺鹏赵可董学会郭艳艳
Owner CHINA AGRI UNIV
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