Automatic decoupling detection method for harmful bacteria intruding into tissues of vegetables or fruits and device

Abstract

The invention discloses an automatic decoupling detection method for harmful bacteria intruding into tissues of vegetables or fruits and a device; when positive currents / reverse currents pass through electromagnets, a sliding trolley stops at the far right side or the far left side, bottom push-and-pull mechanisms arranged on a chassis and a top tray drive a long-wave ultraviolet light emitter to be over against a light-gathering convex lens and drive a photoelectrical detector to detect light intensity of fluorescent lights transmitting upward; a single-chip microcomputer is respectively and externally connected with an electromagnetic valve, a long-wave ultraviolet light emitter, the photoelectric detector, an A / D (analogue-to-digital) switching circuit and a liquid crystal display; anelectric current switching circuit is connected in parallel with tandem analogue switches K1 and K3 and tandem analogue switches K2 and K4 to form a circuit switchover bridge; the two electromagnets are connected in parallel between public passages of the switchover bridge; a control device of the single-chip microcomputer is used for sequentially carrying out steps of escherichia coli detection,total bacterial count detection, comprehensive data processing and displaying; the method realizes the automatic decoupling detection on escherichia coli and salmonella intruding into the interiors of tissues of vegetables; and the control mechanism is simple and has higher control accuracy and synchronism.

Description

technical field [0001] The invention relates to an automatic decoupling detection method and device for harmful bacteria, which can quickly decouple the detection of harmful bacteria intruding into vegetable tissues. Background technique [0002] Fresh vegetables and fruits are often grown in open fields and are exposed to pathogens carried by the soil, irrigation water, manure, wild animals, etc., so there is a risk of bacterial contamination. For fresh fruits and vegetables bought, they are usually sterilized by cleaning. But pathogens like Salmonella and E. coli can get close to the fruit after they are established, and they can move into the vegetable or fruit tissue during fruit growth and ripening, when the plant is pollinated or watered. Therefore, identifying whether the vegetable or fruit tissue is infected by pathogenic bacteria has become a key issue. Since both Escherichia coli and Salmonella are pathogenic bacteria of different diseases, the national food stan...

AI Technical Summary

Problems solved by technology

This method is time-consuming and labor-intensive, and because the shape and size of E. coli and Salmonella are almost the same, the detection results cannot accurately reflect the bacterial infection of vegetables or fruits

Chinese patent application number 200610112410.0 discloses an anti-interference rapid detection method and reagents for the total number of bacteria in food. The method uses ATP hydrolysis fluorescence luminescence to detect the fluorescence intensity, and then combines the luminescence value of the food sample to calculate the ATP concentration and the total number of bacteria in the sample. , although this method realizes the rapid detection of the total number of bacteria, it only has a certain effect on the rapid detection of the total number of bacteria, and it cannot realize the decoupling detection of the harmful bacterial components and the number of each component that invaded vegetables and fruits

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