Formula of culture medium for culturing tobacco anther

Abstract

The invention provides a formula of a culture medium for culturing tobacco anther. The formula comprises the following components in part by weight: 2,800 to 32,000 parts of sucrose, 6,000 to 8,000 parts of agar, 680 to 760 parts of NH4NO3, 880 to 920 parts of KNO3, 130 to 190 parts of CaCl2.2H2O, 150 to 210 parts of MgSO4.7H2O, 60 to 80 parts of NaH2PO4, 50 to 70 parts of Na2-ethylene diamine tetraacetic acid (EDTA), 40 to 50 parts of FeSO4.7H2O, 20 to 30 parts of MnSO4.4H2O, 15 to 25 parts of ZnSO4.7H2O, 15 to 25 parts of H3BO4, 0.3 to 0.7 part of thiamine chloride, 0.3 to 0.7 part of niacin, 0.3 to 0.7 part of pyridoxine hydrochloride, 90 to 110 parts of inositol and 1 to 3 parts of glycine. The pH value is 5.5 to 6.0.

Description

technical field [0001] The invention relates to a culture medium for plant tissue culture, in particular to a medium formula for cultivating tobacco anthers. Background technique [0002] Haploid plants are important materials for genetic research and haploid breeding. At present, anther culture is the most effective method to produce haploid plants. Activated carbon has the effect of generally increasing the emergence rate in anther culture (Bu Xuexian [1998] the adsorption of activated carbon to growth-regulating substances in the medium; Liu Yongsheng [1994] the use of activated carbon in plant tissue culture; Wang Jingju [1983] improved sugarcane tissue Research on culture efficiency; Zhu Zhiqing [1988] Anther and anther culture. Economic plant tissue culture; Jia Xinghua [1991] Tobacco flower cultivation; Cao Ziyi [1996] Practical plant tissue culture technology tutorial; Anagnostakis SL [1974] Haploid plants from anthers of tobacco enhancement with charcoal; Johansson...

AI Technical Summary

Problems solved by technology

Activated carbon has the effect of generally increasing the emergence rate in anther culture (Bu Xuexian [1998] the adsorption of activated carbon to growth-regulating substances in the medium; Liu Yongsheng [1994] the use of activated carbon in plant tissue culture; Wang Jingju [1983] improved sugarcane tissue Research on culture efficiency; Zhu Zhiqing [1988] Anther and anther culture. Economic plant tissue culture; Jia Xinghua [1991] Tobacco flower cultivation; Cao Ziyi [1996] Practical plant tissue culture technology tutorial; Anagnostakis SL [1974] Haploid plants from anthers of tobacco enhancement with charcoal; Johansson L[1982]Improrement of anther culture technique; Fridborg G[1975] Effects of activated charcoal on growth and morphogenesis in cell culture Physiol Plant; Smith RH[1983]In vitro propagation of Nadina domestica. Hortscience, etc.), However, activated carbon has side effects such as preventing the medium from solidifying, increasing the rate of deformed seedlings, and complicating the research (Bu Xuexian [1998] the adsorption of activated carbon to growth-regulating substances in the medium; Liu Yongsheng [1994] the use of activated carbon in plant tissue culture ; Jia Xinghua [1991] Tobacco flower cultivars); especially the increased rate of deformed seedlings, resulting in a decrease in the number of normally available seedlings (Jia Xinghua [1991] Tobacco flower cultivars), sometimes even less than the treatment without activated carbon, for obtaining haploid It is very unfavorable to study for the purpose of somatic plants

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