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Method for extracting and separating pueraria flavonid and pueraria polysaccharide

A technology of Pueraria flavonoids and Pueraria polysaccharides, applied in the direction of organic chemistry, can solve the problems of difficult separation of Pueraria flavonoids and Pueraria polysaccharides, large amount of ethanol, and many impurities, and achieve the effects of convenient operation, increased content, and simple process

Inactive Publication Date: 2011-07-20
WUHAN INSTITUTE OF TECHNOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the cost of enzymatic hydrolysis is high, and only one step of enzymatic hydrolysis results in too many impurities, the separation of Pueraria flavonoids and Pueraria polysaccharides is difficult, and the amount of ethanol is large

Method used

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  • Method for extracting and separating pueraria flavonid and pueraria polysaccharide
  • Method for extracting and separating pueraria flavonid and pueraria polysaccharide

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] 20 g of Pueraria lobata produced in Yunnan, extracted Pueraria flavonoids with 75% ethanol solution (mass ratio), according to the solid-to-liquid ratio of Pueraria lobata (g) and ethanol solution (mL) 1:20, in a 1 L flask, reflux extraction at 80°C 4 h, filter to obtain ethanol extract A and primary filter residue, and then use 75% ethanol solution (mass ratio) to reflux extraction at 80°C for 4 h at a solid-to-liquid ratio of 1:20 for the primary filter residue, and obtain ethanol extract B and the secondary filter residue, and combine it with ethanol extract A to obtain 750 mL of pueraria flavonoids extract; extract the pueraria polysaccharide from the secondary filter residue with water, according to the solid-liquid ratio of filter residue (g) to water (mL) 1:20, 100°C Extract under reflux for 4 h, filter to obtain 380 mL of water extract, and discard the filter residue; concentrate the water extract to obtain 10 mL of water extract concentrate and recover 368 mL of...

Embodiment 2

[0022] 20 g of Pueraria lobata produced in Hubei, extracted Pueraria flavonoids with 80% ethanol solution (mass ratio), according to the solid-liquid ratio of Pueraria lobata (g) and ethanol solution (mL) 1:5, in a 500 mL flask, reflux extraction at 80 °C 1 h, filter to obtain ethanol extract A and primary filter residue, and then use 80% ethanol solution (mass ratio) to reflux extraction at 80°C for 4 h at a solid-liquid ratio of 1:5 for the primary filter residue, and filter to obtain ethanol extract B and the secondary filter residue, the ethanol extract B and the ethanol extract A were combined to obtain 190 mL of Pueraria flavonoids extract; the secondary filter residue was extracted with water, and the solid-liquid ratio of the filter residue (g) to water (mL) was 1:5. Reflux extraction at 100°C for 4 h, filter to obtain 90 mL of water extract, and discard the filter residue; concentrate the water extract to obtain 3 mL of water extract concentrate and recover 86 mL of wa...

Embodiment 3

[0024] 20 g of Pueraria lobata produced in Zhejiang, extracted Pueraria flavonoids with 75% ethanol solution (mass ratio), according to the solid-to-liquid ratio of Pueraria lobata (g) and ethanol solution (mL) 1:10, in a 500 mL flask, reflux extraction at 80 °C 2 h, filter to obtain the ethanol extract A and the primary filter residue, the primary filter residue is then extracted with 75% ethanol solution (mass ratio) according to the solid-to-liquid ratio of 1:20 at 80°C for 2 h, and filtered to obtain the ethanol extract B and secondary filter residue, ethanol extract B and ethanol extract A were combined to obtain 380 mL of kudzu root flavonoids extract; the filter residue after the second extraction of ethanol solution was extracted with water to extract kudzu root polysaccharides, and solidified according to the filter residue (g) and water (mL) Liquid ratio 1:10, reflux extraction at 80°C for 2 h, filter to obtain 190 mL of water extract, discard the filter residue; conc...

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Abstract

The invention relates to a method for extracting and separating pueraria flavonid and pueraria polysaccharide, which comprises the following steps: 1) adding puerariae into ethanol solution to perform primary reflux extraction, filtering to obtain ethanol extract B and secondary filter residue, combining the ethanol extract B and ethanol extract A to obtain pueraria flavonid extract; 2) adding water into the obtained secondary filter residue, performing reflux extraction and filtering to obtain water extract; and 3) concentrating the water extract to obtain water extract concentrate, adding ethanol solution, shaking uniformly, standing, filtering, drying precipitate filtered out to obtain pueraria polysaccharide, mixing the filtrate and the obtained pueraria flavonid extract, concentrating, and drying to obtain pueraria flavonid and recovered ethanol solution. The reagent used in the invention is safe, the operation is convenient, the process is simple, common equipment is used, and the ethanol can be recycled.

Description

technical field [0001] The invention relates to a method for extracting natural products, in particular to a method for extracting and separating pueraria flavonoids and pueraria polysaccharides. Background technique [0002] Pueraria lobata, also known as Luhuo and Jiqi, is the dried root of Pueraria mirifica or Pueraria puerariae. It is a commonly used Chinese medicinal material. It has been recorded in herbal medicines since ancient times. Warming the yang and stopping diarrhea, improving the body's immunity and other functions. At present, kudzu root is widely used to improve cardiovascular and cerebrovascular. In the important compound preparations for the treatment of diarrhea and diabetes, the dosage is extremely large, and it is an important component of the preparations. There are more than 140 kinds of kudzu root compound preparations included in the Pharmacopoeia of the People's Republic of China and the Ministry of Health's Drug Standards, including kudzu root ...

Claims

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Application Information

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IPC IPC(8): C07D311/30C07D311/40C08B37/00
Inventor 池汝安汪洋周芳徐志高
Owner WUHAN INSTITUTE OF TECHNOLOGY
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