Application of bdellovibrio bdelloplast in preparing bacterial agent for preventing and controlling red mouth disease of scophthalmus maximus

A technology of Bdellovibrio and turbot, which is applied in the field of application of Bdellovibrio bdelloplasts in the preparation of antibacterial agents for preventing and treating turbot red-mouth disease, can solve problems such as food safety, achieve effective application, good application prospects, good safety effect

Inactive Publication Date: 2011-05-04
SOUTH CHINA UNIV OF TECH
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  • Abstract
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  • Claims
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Problems solved by technology

[0005] In order to overcome the deficiencies of various food safety problems caused by the abuse of antibiotics in fish farming, the present invention provides an application of a Bdellovibrio bdelloplast in the preparation of a bacterial agent for preventing and treating turbot redmouth disease

Method used

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  • Application of bdellovibrio bdelloplast in preparing bacterial agent for preventing and controlling red mouth disease of scophthalmus maximus
  • Application of bdellovibrio bdelloplast in preparing bacterial agent for preventing and controlling red mouth disease of scophthalmus maximus
  • Application of bdellovibrio bdelloplast in preparing bacterial agent for preventing and controlling red mouth disease of scophthalmus maximus

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Embodiment 1

[0020] The preparation of embodiment 1 bacterial agent

[0021] The Hirudovibrio strain used was Hirudovibrio BDM01. The leech vibrio (Bdellovibriosp.) BDM01 was preserved in the China Center for Type Culture Collection on April 28, 2008, and the deposit number is CCTCC NO: M 208066; after negative staining, the morphology was observed under an electron microscope: leech arc Bacterium BDM01 is single-celled, arc-shaped, 1.7×1.0 μm in size, terminal flagella, and 3.5 μm in length; it can form transparent round phages with a diameter of 3-4 mm when cultured at 28°C for three days by double-layer plate method. Plaque.

[0022] Inoculate 1 mL of a conical flask containing 100 mL of normal nutrient broth (NB) medium (10 g of peptone, 3 g of beef extract, 5 g of sodium chloride, 1000 mL of distilled water, pH 7.4) at a concentration of 10 9 Aeromonas hydrophila (Aeromonas hydrophila, purchased from the Culture Collection Center of Guangdong Institute of Microbiology, No. GIM1.172)...

Embodiment 2

[0024] Select juveniles with healthy physique and uniform size, with an average weight of 55±2.5g, according to 300 fish / m 2 The breeding density was put into the pond, and the experimental period of each breeding pond was 4 months.

Embodiment 21

[0026] The normal running water adopts external drainage, and the amount of water exchange every day and night is 8 running water volumes, and conventional turbot pellet feed (factory-produced breeding method) is put in, and no bacterial agent is added.

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Abstract

The invention discloses application of bdellovibrio bdelloplast in preparing a bacterial agent for preventing and controlling red mouth disease of scophthalmus maximus. The bdellovibrio is (Bdellovibrio sp.) BDM01. The application method for the bacterial agent comprises that: the bacterial agent is put into water body to prevent and control the red mouth disease of scophthalmus maximus to ensure that the final concentration of the bdellovibrio bdelloplast in the water body is 10-10<7>pfu / mL, or a feed is soaked by the bacterial agent for controlling the red mouth disease of scophthalmus maximus for 15 to 45 minutes, and the concentration of the bdellovibrio bdelloplast in the bacterial agent is 10-10<7>pfu / mL. When the bdellovibrio bdelloplast is applied to preparing the bacterial agent for preventing and controlling the red mouth disease of scophthalmus maximus, the bacterial agent can effectively prevent and control the red mouth disease of scophthalmus maximus, has high safety when applied to aquaculture, is environment-friendly, does not cause pathogenic bacteria resistance, and has good application prospect.

Description

technical field [0001] The invention belongs to the field of biotechnology, and relates to the application of fungi, in particular to the application of leech vibrio leech plastid in the preparation of a fungus agent for preventing and treating turbot red-billed disease. Background technique [0002] Turbot (Scophthalmus maximus) is a rare flounder native to Europe. Because it has the advantages of adapting to low water temperature life, fast growth, delicious meat, easy to accept artificial feed, easy to intensive breeding, etc., after it was introduced to China in 1992, it quickly became an important breeding species in the northern coast of China. However, the rapid development and irregular operation of aquaculture have caused the disease problem to become more and more prominent, and there have been many obvious symptoms and large-scale deaths. The main symptoms of red mouth disease are: bleeding from the rostral end and ventral maxilla of the diseased fish, some small...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K35/74A61K9/10A61K9/127C12N1/20A61P31/04
Inventor 蔡俊鹏陈丽芸
Owner SOUTH CHINA UNIV OF TECH
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