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Molecular marker related to chicken carcass characters and acquisition method and application thereof

A technology of molecular markers and carcass traits, applied in the fields of genetic engineering and genetic breeding, can solve the problems that genetic research has not been carried out, and achieve the effect of obvious advantages.

Inactive Publication Date: 2013-11-13
珠海市裕禾农牧有限公司
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Problems solved by technology

However, currently in bird research, the study of this gene has not been carried out

Method used

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  • Molecular marker related to chicken carcass characters and acquisition method and application thereof
  • Molecular marker related to chicken carcass characters and acquisition method and application thereof
  • Molecular marker related to chicken carcass characters and acquisition method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0016] Example Detection and application of FTO gene marker site G+1479A

[0017] 1. Sample collection and DNA extraction

[0018] Use a disposable syringe to draw 1 mL of blood from the vein below the chicken wing, inject it into a 1.5 mL centrifuge tube after autoclaving, record the number of the wing, add about 150 μL of sterile 2% EDTA anticoagulant, shake gently, and store at -20 °C spare.

[0019] Genomic DNA was extracted by conventional phenol / chloroform extraction method. Specific steps are as follows:

[0020] 1) Put 60 μL anticoagulant blood into a 1.5ml centrifuge tube, add 470 μL 1×SET buffer, 12.5 μL 20% SDS, 6 μL 0.01 mg / μL proteinase K, and place in a 55°C water bath overnight for digestion until no blood is lost sticky lumps;

[0021] 2) Add 500 μL of Tirs-saturated phenol to the digested blood, shake gently for 20 minutes to fully mix the two phases of the solution, and centrifuge at 10,000 rpm at 6-8°C for 10 minutes;

[0022] 3) Take the supernatant ...

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Abstract

The invention discloses a molecular marker related to chicken carcass characters and an acquisition method and application thereof. The molecular marker related to the chicken carcass characters is single base mutation of a ninth exon G+1479A positioned at a fat mass and obesity associated (FTO) gene. The molecular marker is obtained by cloning full length of a chicken FTO gene by reverse transcription-polymerase chain reaction (RT-PCR) and rapid-amplification of cDNA ends (RACE) technology, wherein a full-length sequence and a coding region sequence of the FTO gene are shown as SEQIDNO:1-2. Correlation analysis of the chicken carcass characters shows that the carcass weight, wing weight, leg muscle weight, half eviscerate weight and full eviscerate weight of a 90-day-old mutated individual are obviously higher than those of a wild type individual.

Description

technical field [0001] The invention relates to the fields of genetic engineering and genetic breeding, in particular to a molecular marker related to chicken carcass traits and an acquisition method and application thereof. Background technique [0002] Carcass traits are important economic traits in animal production, directly reflecting the meat production performance of animals, such as carcass weight, half-eviscerated weight, eviscerated weight, pectoralis muscle weight and wing weight, etc. research hotspots. On the basis of meat quality, it is the goal of broiler breeding to further increase the carcass weight and leg muscle percentage of broilers. [0003] FTO gene (Fat Mass and Obesity Associated) is a new gene closely related to BMI, obesity and diabetes through genome-wide association analysis in recent years. Human medical research has found that the mutation of FTO is one of the key factors leading to obesity, especially the rs9939609 mutation site located in ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/11C12N15/10C12Q1/68
Inventor 聂庆华贾新正欧阳宏佳张细权
Owner 珠海市裕禾农牧有限公司
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