Preparation method and discrimination method of coating seeds with anti-counterfeit function
A technology for coating seeds and functions, which is applied in the field of preparation and identification of coated seeds with anti-counterfeiting function, can solve the problems of confusion of anti-counterfeiting products and imitation of anti-counterfeiting costs, and achieves the effects of rapid detection, reduced possibility and low cost.
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preparation example Construction
[0017] The steps of the preparation method of the coated seed with anti-counterfeiting function are as follows:
[0018] 1) Na 2 HPO 4 2H 2 O 35.61g or Na 2 HPO 4 ·7H 2 O 53.65g or Na 2 HPO 4 12H 2 Dissolve O71.7g in 800ml distilled water, then dilute to 1000ml with distilled water, and prepare 0.2mol / LNa 2 HPO 4 Solution, namely mother liquor A; NaH 2 PO 4 ·H 2 O 27.6g or NaH 2 PO 4 2H 2 O 31.2g was dissolved in 800ml of distilled water, and then the volume was adjusted to 1000ml with distilled water to prepare 0.2mol / LNaH 2 PO 4 Solution, i.e. mother liquor B;, mix mother liquor A (x ml) with mother liquor B (y ml) and then dilute to 200ml with distilled water to obtain phosphate buffered solution with a concentration of 0.1mol / L and different pH values as shown in the table below liquid:
[0019] x(ml)
y(ml)
pH
72.0
28.0
7.2
77.0
23.0
7.3
81.0
19.0
7.4
84.0
16....
Embodiment 1
[0025] Add 1.5 g of fluorescein to 500 ml of 0.1 mol / L phosphate buffer with pH 8.0, adjust the stirrer to 600 rpm, and stir until a uniform fluorescent solution is formed, with a concentration of 3.0 mg / ml. Weigh 100 g of rapeseed seeds, spray with the prepared solution when coating the inner layer powder, and perform seed coating treatment according to the conventional method to obtain coated seeds with anti-counterfeiting function. Place the obtained coated seeds directly on a glass slide, with an interval of more than 1 cm between the coated seeds, and then use a dropper to absorb a pH 8.0 phosphate buffer solution with a concentration of 0.1 mol / L, and drop 1 to 2 drops on each seed. After 5 seconds, place the glass slide under ultraviolet light with a wavelength of 200-400nm, and the bright yellow fluorescence can be observed with the naked eye. The effects of treatment on seed germination and seedling growth are as follows (Table 1):
[0026] Table 1 Germination and se...
Embodiment 2
[0030] Add 0.5 g of fluorescein to 500 ml of 0.1 mol / L phosphate buffer with pH 7.4, adjust the stirrer to 500 rpm, and stir until it becomes a uniform fluorescent solution with a concentration of 1.0 mg / ml. Weigh 100g of onion seeds, spray with the prepared solution when coating the inner layer powder, and coat the seeds according to the conventional method to obtain the coated seeds with anti-counterfeiting function. Place the obtained coated seeds directly on a glass slide, with an interval of more than 1 cm between the coated seeds, and then use a dropper to absorb a pH 8.0 phosphate buffer solution with a concentration of 0.1 mol / L, and drop 1 to 2 drops on each seed. After 5 seconds, place the glass slide under ultraviolet light with a wavelength of 200-400nm, and the bright yellow fluorescence can be observed with the naked eye. The effect of treatment on seed germination and seedling growth is as follows (table 2):
[0031] Table 2 Germination and seedling growth perf...
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