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Simultaneous detection method of fluoroquinolones medicines and chloramphenicols medicines in food

A fluoroquinolone and chloramphenicol technology, which is used in measuring devices, instruments, material analysis by electromagnetic means, etc., can solve the problems of many interference factors, insufficient sensitivity, cross-reaction, etc., and achieves simple pretreatment and analysis time. Short, repeatable results

Inactive Publication Date: 2012-07-25
中华人民共和国珠海出入境检验检疫局
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Problems solved by technology

However, this method is not sensitive enough at lower limit levels, and because there are many types of antibiotics with antibacterial effects, there are many interference factors, and the false positives are high.
Immunoassay technology is highly sensitive, fast and simple, but there are problems of cross-reaction and false positive

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  • Simultaneous detection method of fluoroquinolones medicines and chloramphenicols medicines in food
  • Simultaneous detection method of fluoroquinolones medicines and chloramphenicols medicines in food
  • Simultaneous detection method of fluoroquinolones medicines and chloramphenicols medicines in food

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Embodiment Construction

[0049] The detection method of the present invention is mainly used to detect 13 kinds of fluoroquinolones and 3 kinds of chloramphenicols in food, wherein, 13 kinds of fluoroquinolones are ciprofloxacin, danofloxacin, difloxacin , enrofloxacin, lomefloxacin, marbofloxacin, nalidixic acid, norfloxacin, ofloxacin, oxolinic acid, pefloxacin, sarafloxacin, flumequine, 3 kinds of chloramphenicol The steroid drugs are chloramphenicol, thiamphenicol, and fluthiamphenicol. In this specification, the scanning images of MS / MS are all scanning images of EPI.

[0050] [Detection process]

[0051] see figure 1 The detection method of the present invention comprises the following steps:

[0052] 1. Sample processing

[0053] Extraction: Accurately weigh 5.0g sample (accurate to 0.01g) into a 50mL centrifuge tube, add 100μg / L isotope-labeled chloramphenicol internal standard (Chloramphenicol-D 5 ) solution and 1000 μg / L isotope-labeled ciprofloxacin internal standard (Ciprofloxacin-D ...

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Abstract

The invention builds a liquid chromatograph-mass spectrometer database of 13 types of floxacins medicines and 3 types of chloramphenicols medicines, and an LC-MS / MS detection method. The method has the advantages of simple preprocessing and good repeatability. Combining with the chromatograph-mass spectrometer database built in the invention, the method can realize the rapid quantitative and qualitative analysis of 13 types of the fluoroquinolones medicines and 3 types of the chloramphenicols medicines, the analysis time is shortened by a half, and the method is extremely suitable for the import and export rapid confirmation work.

Description

【Technical field】 [0001] The present invention relates to the field of food safety, and relates to a method for simultaneously detecting 13 fluoroquinolones and 3 chloramphenicols in food and a method for establishing 13 fluoroquinolones and 3 chloramphenicols Methods for liquid mass spectral libraries. 【Background technique】 [0002] Fluoroquinolones (fluoroquinolones) are the third-generation quinolones, which selectively inhibit bacterial DNA-gyrase, thereby inhibiting bacterial DNA synthesis, resulting in DNA degradation and death. This type of drug has a broad antibacterial spectrum, high efficiency, low toxicity, and strong tissue penetrating ability. The antibacterial effect is nearly a thousand times that of sulfonamides and comparable to the third-generation cephalosporin antibiotics. It also has a good preventive effect on the digestive tract and gill bacterial diseases of aquatic fish. Since the 1980s, it has become the main antibacterial drug for the treatment o...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/02G01N30/06G01N27/62
Inventor 冯家望蔡勤仁周新朋梁玉英钱振杰彭玉芬蔡杰薛良辰
Owner 中华人民共和国珠海出入境检验检疫局
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