Rooting method of casuarina equisetifolia sterile test-tube seedling

Abstract

The invention discloses a rooting method for a casuarina equisetifolia sterile test-tube seedling, which comprises the following steps of: cutting a fresh cut on the base part of the sterile test-tube seedling, inserting the base part of the sterile test-tube seedling into a sterilized and cooled mesenchymal stem cell (MSC) liquid culture medium which contains 20 to 30 mum of naphthylacetic acid and 15 to 25g / L of saccharose and culturing for 2 to 3 days under the conditions of a temperature of 27+ / -3 DEG C, an illumination time period of 12 to 20 hours each time and illumination intensity of45 to 60 mu mol / m<2> / s; taking out the sterile test-tube seedling and washing the base part of the sterile test-tube seedling with sterilized de-ionized water for 2 to 5 times; and transferring the sterile test-tube seedling into the sterilized and cooled MSC liquid culture medium which contains 30 to 50g / L of saccharose for cultivation for 1 to 2 weeks under the condition of a temperature of 27+ / -3 DEG C, an illumination time period of 12 to 20 hours each time and illumination intensity of 45 to 60 mu mol / m<2> / s. The rooting method for the casuarina equisetifolia sterile test-tube seedling has the advantages that: rooting is quick and efficient; roots can grow after 10 days of cultivation; rooting efficiency is high and reaches 100 percent after two weeks of cultivation; a large number of roots grow; and the roots extend and grow obviously.

Description

Technical field [0001] The present invention belongs to the field of non -propagation technology of plants. Specifically, the invention involves a rooting method of wooden ephedra sterile test tube seedlings. Background technique [0002] Wooden ephedra is a tree species with important ecology, social and economic benefits, and researching and developing the tree species has global impact.At present, the area of wooden ephedra artificial forests in the world's tropical and subtropical regions is about 2 million hectares.Wooden ephedra is the main tree species for the construction of coastal protective forests. It is also a pioneer tree species recovered by farmland protective forests and degenerate vegetation. Its wood can produce column materials, carbon materials, plate materials and pulp.At present, the planting area of ​​wooden eloquent artificial forests in my country has reached more than 300,000 hectares.Wooden Ephedra played an irreplaceable role in wind and sand and vege...

AI Technical Summary

Problems solved by technology

[0006] Therefore, someone proposed to take the aseptic test-tube plantlets directly to the greenhouse for hardening without roots, and then use the traditional hydroponics method to cultivate and take root. The test found that the rooting rate of this method is very low, and the aseptic test-tube plantlets are easy to grow without roots. dead before

There are also researchers (Duhoux et al., 1990; Parthiban et al., 1996) to induce rooting of aseptic test-tube plantlets in solid medium supplemented with substances such as hormones and activated carbon, but the rooting rate is not high, the time for inducing rooting is longer, and it is easy to induce Callus produced from base wound of sterile test-tube plantlets

In addition, some researchers have replaced the solid medium usually used for Casuarina equisetifolia tissue culture with liquid medium to induce rooting culture of sterile test-tube plantlets. The rooting rate can reach about 50%, but the elongation and growth of roots are inhibited. The growth is slow, and the wound will also produce callus, and the effect is also unsatisfactory

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