Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Chinese cabbage EST-SSR labeled primers and application thereof to identification of varieties

A technology for labeling primers and Chinese cabbage, which is applied in the field of molecular biology and can solve problems such as unsatisfactory development

Active Publication Date: 2010-12-15
VEGETABLE RES INST OF SHANDONG ACADEMY OF AGRI SCI
View PDF0 Cites 7 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the development of Chinese cabbage EST-SSR primers is far from being saturated

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Chinese cabbage EST-SSR labeled primers and application thereof to identification of varieties
  • Chinese cabbage EST-SSR labeled primers and application thereof to identification of varieties
  • Chinese cabbage EST-SSR labeled primers and application thereof to identification of varieties

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0037] 1. Development of Chinese cabbage EST-SSR marker primers

[0038] The Chinese cabbage EST sequence used to develop primers comes from GenBank (http: / / www.ncbi.nlm.nih.gov), and as of October 31, 2007, there is a total of Chinese cabbage (B.rapa ssp.pekinensis) EST sequence 147217 indivual. In this study, 147217 Chinese cabbage EST sequences were used to develop SSR primers.

[0039] The downloaded Chinese cabbage EST sequence was pre-processed with reference to the method in Ge Jia et al. (see "Journal of Agricultural Biotechnology", No. 04, 2005, "Analysis of Chinese Cabbage Expressed Sequence Tag SSR Marker"), including removing the EST sequence. Existing vector sequence 5' end polyT and 3' end polyA sequence and sequences containing ambiguous bases, remove the sequence less than 100bp. Contigs were then constructed on the processed EST sequences using the SeqMan program (Lasergene software package, DNAStar Inc.). Use MISA software (http: / / www.pgrc.ipk-gatersleben....

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to Chinese cabbage EST-SSR labeled primers and an application thereof to identification of varieties, belonging to the technical field of molecular biology. The invention provides sequences of a Chinese cabbage EST-SSR labeled primer BRE28, a Chinese cabbage EST-SSR labeled primer BRE121 and a Chinese cabbage EST-SSR labeled primer BRE131, and an application of the labeled primers to identification of varieties of Chinese cabbages. The amplified products of the EST-SSR primers of the invention are in parental complementary banding patterns which have obvious size difference, thus hybrids and parental inbred lines can be well distinguished, and different varieties of the Chinese cabbages can be identified. The amplified products among individuals in the same variety have consistent banding patterns.

Description

technical field [0001] The invention relates to a Chinese cabbage EST-SSR marker primer and its application in variety identification, belonging to the technical field of molecular biology. Background technique [0002] Chinese cabbage (Brassica rapa L.ssp.pekinensis) is a Brassica plant in the family Brassicaceae, and it is an important vegetable in my country and East Asian countries. Variety differences directly affect the production and eating quality of Chinese cabbage, so the identification of Chinese cabbage varieties and purity identification are very important. The most reliable traditional testing method for seed purity is the GOT (grow-out test) method, which uses field planting to identify hybrids based on morphological characteristics. The biggest disadvantage of the GOT method is that it is time-consuming and labor-intensive, and it is easily affected by environmental and subjective factors. In recent years, isoenzymes and protein electrophoresis can be used ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12Q1/68C12N15/11
Inventor 高建伟李利斌周新成刘立锋李化银张庶
Owner VEGETABLE RES INST OF SHANDONG ACADEMY OF AGRI SCI
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com