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Lactobacillus used for biological preservation and application thereof

A technology of biological preservation and lactic acid bacteria, applied in the field of lactic acid bacteria, can solve the problems of teratogenicity and easy to cause food poisoning

Active Publication Date: 2011-08-24
山东宝来利来生物工程股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Long-term studies have shown that chemically synthesized preservatives have carcinogenicity, teratogenicity, and food poisoning; natural preservatives are not only harmless to human health, but also have certain nutritional value, so more and more researchers Start to focus on the development of natural preservatives

Method used

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  • Lactobacillus used for biological preservation and application thereof
  • Lactobacillus used for biological preservation and application thereof
  • Lactobacillus used for biological preservation and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0198] Embodiment 1 prepares a kind of antiseptic and fresh-keeping preparation

[0199] 1. Strain preparation method:

[0200] (i) Strain: Lactobacillus plantarum was selected, CCTCC M 2010150;

[0201] (ii) Slant culture: inoculate the freeze-dried powder strains on a solid slant medium, and culture at 37°C for 24 hours;

[0202] (iii) Primary seed culture: Put the cultured slant into 100mL seed liquid culture medium with an inoculation loop under sterile conditions, and culture it statically for 14 hours at 37°C to obtain the primary seed liquid ;

[0203] (iv) Expanded cultivation: with an inoculum amount of 5%, the primary seed solution was placed in 1000 mL of seed liquid medium, and cultured at 37° C. for 10 hours to obtain a secondary seed solution;

[0204] (v) Fermentation tank culture: with an inoculum amount of 5%, the secondary seed liquid was placed in a liquid fermentation medium, and cultured statically for 18 hours at 37° C.;

[0205] (vi) Collect the ferm...

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Abstract

The invention discloses lactobacillus used for biological preservation, which is named Lactobacillus plantarum and has been collected in China Center for Type Culture Collection on June 21, 2010 with the collection number of CCTCC M 2010150. The lactobacillus can be used for fruit preservation. The application method comprises the following steps: heating and dissolving an acidizer in water, reducing temperature to normal temperature, adding lactobacillus powder, uniformly mixing, spraying onto fruits in an atomized mode, and storing the fruits at normal temperature or low temperature. Under the condition of normal temperature, the fruits can be preserved for 3 months, and under the condition of low temperature, the fruits can be preserved for 6 months. The Lactobacillus plantarum is obtained by screening a large amount of lactobacillus, can control peel browning and reduce fruit morbidity, and also can properly reduce fruit moisture violation, inhibit the metabolism of the fruits, and prolong the refreshing time.

Description

technical field [0001] The invention relates to a lactic acid bacterium which can be used for biological antisepsis and preservation and its application in fruit antisepsis and preservation. Background technique [0002] Currently, postharvest fruit loss is a global problem that has attracted great attention worldwide. About 80 million tons of vegetables and fruits rot every year in my country, and the post-harvest loss rate of fruits and vegetables reaches 20% to 40%, with a total loss value of nearly 80 billion yuan. [0003] Worldwide, 25% of fresh fruit products cannot be used due to rot and deterioration during storage. Some perishable fruits have a loss of more than 30% after harvest. In 1992, my country's fruit output reached 24.4 million tons, and it is estimated that the loss due to postharvest diseases reached 6.1 million tons. In 1998, the total fruit output reached 54.529 million tons, with an estimated loss of 13.63 million tons. In recent years, with the ad...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A23B7/155C12R1/25
Inventor 单宝龙谷巍孙合美徐海燕吴琦琦张彩芳
Owner 山东宝来利来生物工程股份有限公司
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