Cabbage haploid breeding method

A technique of haploid and haploid induction, applied in horticultural methods, botanical equipment and methods, plant gene improvement, etc., can solve the problems of long and low efficiency of cabbage breeding methods, and achieve the goal of speeding up and avoiding hybrids Effect

Inactive Publication Date: 2010-11-24
深圳市湖尔美农业生物科技有限公司
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Problems solved by technology

[0003] The purpose of the present invention is to overcome the shortcomings of the long and low efficiency of the existing Chinese cabbage breeding methods, and provide a method for rapidly selecting new varieties of Chinese cabbage, that is, a method for haploid breeding of Chinese cabbage

Method used

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Embodiment Construction

[0083] Below in conjunction with specific embodiment the method for haploid breeding of Chinese cabbage of the present invention is described in further detail, and this method comprises the following steps:

[0084] 1) Preparation of explants: According to different breeding objectives, the preparation of explants is also different: for conventional new varieties or new hybrid varieties male line selection, select complementary breeding materials that meet the breeding objectives The buds are emasculated and crossed to obtain the first generation of hybrids, and the first generation of hybrids is sown in stages, and the anthers are taken as explants after the bolting and flowering. For the breeding of male sterile lines, that is, the female parent lines of new hybrid varieties, the existing male sterile maintainer lines that meet the breeding objectives are selected and crossed with corresponding complementary breeding materials to obtain the first generation of hybrids. The ...

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Abstract

The invention discloses a cabbage haploid breeding method, particularly a method for quickly breeding new cabbage variety, aiming at overcoming the defects that the traditional cabbage breeding method results in long process and low efficiency. The method utilizes the cabbage haploid culturing technology and the traditional male sterile line breeding technology to complete the quick breeding of the good and pure cabbage line. The method comprises the following steps of: preparing an explant, inducing the haploid, regenerating and culturing, transplanting to the field, doubling the chromosome, isolating and pollinating, sieving economic character, and subsequent breeding. The invention adopts the cabbage anther haploid breeding technology to breed and omits the insemination process, thereby avoiding generating heterozygous, simplifying the homozygous procedure of the heterozygous, greatly shortening the breeding period, and enhancing the breeding efficiency.

Description

Technical field: [0001] The invention relates to Chinese cabbage breeding technology, in particular to a method for haploid breeding of Chinese cabbage, which is used for rapid selection of new varieties of Chinese cabbage. Background technique: [0002] At present, in the field of Chinese cabbage breeding, the method of strain selection is mainly used to select new pure lines of Chinese cabbage. The strain screening method is to artificially peel and emasculate the complementary breeding materials that meet the breeding objectives to obtain F 1 The first generation is the hybrid generation, and then the F 1 Multi-generation single-plant selfing is carried out, and the economic characteristics of the single-line materials of each generation are compared, and excellent single plants are screened out for the next generation of single-plant selfing, so as to breed the new pure cabbage that basically meets the requirements. Tie. Although the strain screening method can select...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00A01H1/02A01H1/08
Inventor 徐代安胡璇易琼张更鑫吕绍庄将启安
Owner 深圳市湖尔美农业生物科技有限公司
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