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Application of rice OsEATB gene in modifying rice yield traits

A rice, gene technology, applied in the field of transgenic

Inactive Publication Date: 2010-11-17
FUDAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, the biological functions of AP2 are almost only related to the response of plants (or crops) to stress and related regulatory routes, and there are no reports about the formation and improvement of yield traits of AP2.

Method used

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  • Application of rice OsEATB gene in modifying rice yield traits
  • Application of rice OsEATB gene in modifying rice yield traits
  • Application of rice OsEATB gene in modifying rice yield traits

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0052] 1. Select the full-length ORF segment of OsEATB (see SEQ ID NO.1 for its full-length cDNA sequence, and SEQ ID NO.2 for its coding sequence), amplify it from indica rice 9311 by PCR method, and synthesize an insert linker to clone it into a plant The expression vector pCAMBIA1304 was used to obtain the OsEATB expression vector pCAMBIA1304 / lrk1.

[0053] 2. Culture medium for inducing rice callus

[0054] (1) Induction and subculture medium: MS+2mg / L 2,4-D.

[0055] (2) Hyperosmotic medium: MS+2mg / L 2,4-D+46.67g / L sorbitol+46.67g / L mannitol.

[0056] (3) The first round of screening medium: MS+2mg / L 2,4-D+30mg / L hygromycin.

[0057] (4) The second round of screening medium: MS+2mg / L 2,4-D+50mg / L hygromycin.

[0058] (5) Differentiation medium: MS+3mg / L 6-BA+0.5mg / L NAA+50mg / L hygromycin.

[0059] (6) Medium for rooting and strong seedlings: 1 / 2MS+0.1mg / L NAA.

[0060] (Note: 1. The above mediums all contain 30g / L sucrose + 2.5g / L agar, pH 5.8. 2. The culture conditi...

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Abstract

The invention belongs to the field of transgenic technology, and particularly relates to application of rice OsEATB gene in modifying rice yield traits. In the invention, rice AP2 transcription factor OsEATB gene and homologous gene thereof are used to transform subspecies japonica rice and nonglutinous rice so as to modify the yield traits of the rice; in addition, the rice AP2 transcription factor OsEATB gene and the homologous gene thereof are also used to transform corns, wheat, broomcorn and other cereal crops so as to modify the yield traits of the cereal crops; furthermore, OsEATB gene and homologous gene thereof are used to transform dicotyledon so as to modify the yield traits of the dicotyledon, including the increase of branch number, grains, fruit number and volume and weight of the grains and the fruits.

Description

technical field [0001] The invention belongs to the technical field of transgenics, and in particular relates to a method for improving yield traits of rice. Background technique [0002] Rice is one of the most important food crops in the world, and about 2 / 3 of the world's population uses rice as a staple food. In order to meet the growing population's demand for food, it is of great significance to increase rice productivity. Yield traits are important agronomic traits to describe rice productivity, mainly composed of effective panicle number, grain number per panicle and thousand-grain weight. These yield traits belong to quantitative traits (QTL), which are controlled by many genes with different effects. [0003] At present, the technology of using transgenes to improve the economic traits of crops at home and abroad is mainly based on the known relationship between functional genes and phenotypic traits, using constitutive expression vectors or tissue-specific expre...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/29C12N15/82A01H5/00
Inventor 祁巍巍孙凡杨金水
Owner FUDAN UNIV
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