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Method for establishing RFLP characteristic map of red tide algae

A red tide algae and map technology, applied in biochemical equipment and methods, microbial determination/inspection, etc., can solve problems such as loss of offshore aquaculture, hinder respiration, etc., and achieve the effect of rapid and simple preliminary identification

Inactive Publication Date: 2010-10-20
OCEAN UNIV OF CHINA
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Problems solved by technology

Finally, the mucus produced by some red tide organisms (such as certain dinoflagellates) adheres to the gills of marine animals, hinders respiration, suffocates large aquatic animals, and causes huge losses to the offshore aquaculture industry

Method used

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  • Method for establishing RFLP characteristic map of red tide algae
  • Method for establishing RFLP characteristic map of red tide algae
  • Method for establishing RFLP characteristic map of red tide algae

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Embodiment Construction

[0046] The invention provides a method for establishing a red tide algae RFLP characteristic map, wherein, comprising the following steps:

[0047] a. Establishment of simulation experiment data table

[0048] b. Electrophoretic spectrum of simulated enzyme digestion

[0049] c. Control experiments of simulated digestion and actual digestion

[0050] d. Actual enzyme digestion experiment

[0051] e. Simulated enzyme digestion experiment

[0052] f. Comparison of simulated enzyme digestion experiment and actual enzyme digestion experiment results

[0053] g. Optimization of enzyme digestion experiment conditions

[0054] h. Establishment of RFLP map

[0055] i. Identification of red tide algae species based on RFLP technology.

[0056] The step a is: input the sequence information of the experimental algal species into the excel software, and combine the restriction endonuclease digestion information used in the experiment, and use the excel software to make the informati...

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Abstract

The invention discloses a method for establishing a RFLP characteristic map of red tide algae, which comprises the following steps: a, the establishment of simulated experimental data tables; b, simulated enzyme digestion electrophoretogram; c, simulated enzyme digestion and actual enzyme digestion control experiments; d, an actual enzyme digestion experiment; e, a simulated digestion experiment; f, contrast between results of the simulated digestion experiment and the actual enzyme digestion experiment; g, the optimization of digestion experiment conditions; h, the establishment of RFLP map; and i, the identification of red tide algae species based on RFLP technology. The invention is used as a molecular ecology means for identifying the red tide algae species, and can be used as a complementary and auxiliary means of the traditional identification method. Based on FELP, the invention establishes a characteristic map, standardizes the entire process, and can be favorable for rapid and simple preliminary identification of the red tide algae species.

Description

Technical field: [0001] The invention relates to the technical field of marine phytoplankton species and / or quantity detection, in particular to a method for establishing a red tide algae RFLP characteristic map. Background technique: [0002] In the rich ocean world, there are many marine creatures. In addition to various marine fish and other marine animals, there are also marine plants that provide basic productivity for the ocean. They are distributed in all levels of the ocean, and marine algae, which are the main components of marine plants, are a precious natural wealth of human beings. They can be used as food and health medicines. According to the different living habits of marine algae, people divide them into two types: benthic algae and planktonic algae. [0003] Phytoplankton consists of only one cell, also known as unicellular algae, which is a type of autotrophic unicellular plankton that has pigments or pigment bodies that can perform photosynthesis and prod...

Claims

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Application Information

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IPC IPC(8): C12Q1/68C12Q1/04
Inventor 米铁柱张涛甄毓于志刚
Owner OCEAN UNIV OF CHINA
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