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Dendrobium devonianum clone seedling reproduction method

A Dendrobium dendrobium clone technology, applied in the field of Dendrobium seedling propagation, can solve the problems of difficulty in maintaining the excellent traits of the mother plant, lack of consistency and stability of seedlings, and differences in provenance, so as to maintain a high degree of consistency and Stability, conducive to factory production, and the effect of speeding up the process

Inactive Publication Date: 2013-01-23
YUNNAN DEHONG TROPICAL AGRI RES INST
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Problems solved by technology

[0002] Dendrobium is a traditional precious Chinese medicinal material in my country, and Dendrobium dendrobium is one of them. At present, the cultivation of Dendrobium dendrobium seedlings is mainly based on seeds as explants, and tissue culture techniques are used to obtain seedlings. It is basically mature, and the sexual reproduction system of seeds has been established, but due to the significant differences between the provenances, the seedlings lack consistency and stability, and it is more difficult to maintain the excellent traits of the mother plant

Method used

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Embodiment Construction

[0006] Get the high-quality single plant of Dendrobium dendrobium as material, and use the stem section as the explant. The specific method includes the following steps: (1) explant disinfection: select the stem section, sterilize the surface with 75% alcohol for 30 to 60 seconds, and then Disinfect it in 0.1% mercuric chloride solution for 10 to 20 minutes, then rinse it with sterile water for 5 to 6 times; (2) induction culture: inoculate the stem segments on the induction medium, which is MS+6 -BA 2-4 mg / L + NAA 0.01-0.05 mg / L + coconut milk 10-50 ml / L + ascorbic acid 0.01-0.05 mg / L + potato juice 20-50 g / L + sucrose 20-40 g / L 1, the culture time is 35-40 days, and many single buds can be taken out at the stem nodes at this time; (3) Proliferation culture: the single buds are cut off from the stem and transferred to the proliferation medium, and the proliferation medium is MS+ 6-BA 2-4 mg / L + NAA 0.1-1 mg / L + coconut milk 10-50 ml / L + ascorbic acid 0.01-0.05 mg / L + potato j...

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Abstract

The invention discloses a dendrobium devonianum clone seedling reproduction method and relates to the technical field of bioengineering. In the technical scheme of the reproduction method, a high-quality single dendrobium devonianum plant is used as raw material; a stalk section is used as an explant; induction culture, propagation culture and rooting culture are carried out after disinfection; and in every culture phase, temperature is controlled to be 25+ / -2 DEG C; illumination intensity is controlled to be 1500-2000 lx; illumination time is 12h / day; and environmental noise is less than 40db. The method not only enables a seedling to maintain excellent shape and properties of a parent plant, but also improves seedling reproduction coefficient, thereby laying a foundation for industrialized production of dendrobium devonianum.

Description

technical field [0001] The present invention relates to the propagation method of dendrobium nodulum seedlings, more specifically, the present invention relates to the propagation method of dendrobium dendrobium clonal seedlings. Background technique [0002] Dendrobium is a traditional precious Chinese medicinal material in my country, and Dendrobium dendrobium is one of them. At present, the cultivation of Dendrobium dendrobium seedlings is mainly based on seeds as explants, and tissue culture techniques are used to obtain seedlings. It is basically mature, and the sexual reproduction system of seeds has been established, but due to the significant differences among the provenances, the seedlings lack consistency and stability, and it is more difficult to maintain the excellent traits of the mother plant. Contents of the invention [0003] In order to overcome the deficiencies in the existing Dendrobium dendrobium seedling propagation technology, the present invention pro...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 李守岭白燕冰李泽生耿秀英
Owner YUNNAN DEHONG TROPICAL AGRI RES INST
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