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A method for establishing the correlation between lignin content and fiber quality in cotton fiber

A technology of cotton fiber and correlation relationship, applied in the field of biochemical analysis, can solve the problems of low lignin content of cotton fiber, which cannot be directly pushed and applied to the detection of lignin content, so as to reduce acid loss, control test error, and quantify The effect of precision

Inactive Publication Date: 2011-12-28
新疆农业科学院核技术生物技术研究所
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Problems solved by technology

However, due to the low lignin content of cotton fiber, this method cannot be directly applied to the detection of lignin content in cotton fiber.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] 1. Instruments and reagents

[0016] Autoclave, constant temperature and constant temperature drying oven, HVI SPECTRUM-I cotton fiber quality detector.

[0017] Tris-HCl (Beijing Xinjingke Company); Triton x-100 analytically pure (Beijing Xinjingke Company); NaCl analytically pure (Xi’an Chemical Reagent Factory); acetone analytically pure (Zhengzhou Dezhong Chemical Reagent Factory); concentrated Analytical pure sulfuric acid (Tianjin Shengmiao Fine Chemical Co., Ltd.).

[0018] Homogenization buffer (50 mM Tris-HCl, 1% Triton x-100, 1M NaCl, pH 8.3).

[0019] 72% sulfuric acid (w / w), stored in refrigerator at 4°C.

[0020] 2. Cotton sample collection and fiber quality determination

[0021] Collect 40 natural mature cotton fiber samples in the field, pick out non-cotton fiber impurities such as leaves and seed shells mixed in the cotton fibers, and use the HVI SPECTRUM-I cotton fiber quality detector to measure cotton fiber quality indicators: fiber length, ratio ...

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Abstract

The invention provides a method for establishing the correlation between lignin content in cotton fiber and fiber quality. The traditional Klason method was improved to measure the lignin content in cotton fiber, and then the correlation and regression equation between lignin content and fiber length, specific strength and micronaire value were established. The evaluation method of fiber quality is enriched, and it has positive significance for guiding cotton breeding and production.

Description

1. Technical field [0001] The invention belongs to the field of biochemical analysis, and relates to a fiber quality detection and analysis method, in particular to a method for establishing the correlation between lignin content and fiber quality in cotton fibers. 2. Background technology [0002] Cotton fibers are formed by ovule epidermal cells through differentiation, protrusion, elongation, secondary wall thickening and dehydration maturation before and after fertilization. Scholars at home and abroad believe that mature cotton fibers do not contain lignin that forms the cross-linked structure of the cell wall. The method proves that mature cotton fibers contain lignin that forms cell wall cross-linked structures. It is also proposed that lignin is an important component of the cotton fiber cell wall and forms a cross-linked structure with polysaccharides in the cell wall, which affects the growth, strength, morphogenesis and resistance to biotic and abiotic stress of ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N5/04
Inventor 胡文冉范玲师维军闫洪颖杨洋李波马君吾买尔江
Owner 新疆农业科学院核技术生物技术研究所
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