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Method for extracting polysaccharides from fishbone of hairtail

A fish bone and hairtail technology, which is applied in the field of polysaccharide extraction from hairtail fish bones, can solve the problems of slow enzymatic hydrolysis, long reaction time, and low product yield, and achieve easy operation, reduced enzyme consumption, and reduced production costs Effect

Inactive Publication Date: 2011-07-20
SHANDONG MEIJIA GROUP
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The disadvantages of this method are: slow enzymatic hydrolysis, large amount of enzyme, long reaction time, multiple dissolution and re-precipitation methods for purification, large amount of alcohol, and low product yield

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Take 150g of fresh hairtail fish bones, remove the flesh from the fish bones, and boil them in water at 90°C for 90 minutes to fully remove the fat in the fish bones, then clean and crush the fish bones until all the fish bone particles pass through a 30-mesh sieve.

[0025] Weigh 100g of pulverized fresh hairtail bones, add 200ml of water, add 200mmol sodium hydroxide, mechanically stir at 1500r / min for 360min, adjust the pH to 8.5 with dilute hydrochloric acid, add trypsin 50U / g fishbone and 0.3g calcium chloride, react at 50°C for 180min, adjust the pH value to 6.5, then raise the temperature to 85°C to inactivate the enzyme, filter, adjust the pH of the filtrate to 11, add 3ml H 2 o 2 , oxidation treatment for 90min, adjust the pH to 6.5, add 6g Ca 3 (PO 4 ) 2 , stirred mechanically at 1500r / min for 120min, filtered the filtrate, added alcohol with a volume fraction of 95% to the filtrate, and kept stirring so that the volume content of alcohol in the solution wa...

Embodiment 2

[0027] Take 150g of fresh hairtail fish bones, remove the flesh from the fish bones, and boil them in water at 100°C for 10 minutes to fully remove the oil in the fish bones, then clean the fish bones and crush them until all the fish bone particles pass through a 30-mesh sieve.

[0028] Weigh 100g of crushed fresh hairtail bones, add 300ml of water, add 900mmol of potassium hydroxide, mechanically stir at 1500r / min for 240min, adjust the pH to 7.0 with dilute hydrochloric acid, add neutral protease 80U / g fishbone while stirring React with 0.4g calcium acetate for 240min at a temperature of 70°C, adjust the pH value to 6.5, then raise the temperature to 85°C to inactivate the enzyme, filter, adjust the pH of the filtrate to 11, add 4ml H 2 o 2 , oxidize for 90min, adjust the pH to 6.5, add 8g Ca3(PO4)2, stir mechanically at 1500r / min for 120min, filter the filtrate, add alcohol with a volume fraction of 95% in the filtrate, and keep stirring to make the solution The volume co...

Embodiment 3

[0030] Take 150g of fresh hairtail fish bones, remove the flesh from the fish bones, and boil them in water at 90°C for 180 minutes to fully remove the fat in the fish bones, then clean the fish bones and crush them until all the fish bone particles pass through a 30-mesh sieve.

[0031] Weigh 100g of crushed fresh octopus bones, add 300ml of water, add 200mmol of sodium hydroxide, mechanically stir at 1500r / min for 420min, adjust the pH to 8.5 with dilute hydrochloric acid, add alkaline protease 70U / g fishbone while stirring and 0.4g calcium chloride, reacted at a temperature of 55°C for 240min, adjusted the pH value to 6.5, then raised the temperature to 85°C to inactivate the enzyme, filtered, adjusted the pH of the filtrate to 11, added 4ml H 2 o 2 , oxidation treatment for 90min, adjust the pH to 6.5, add 8g Ca 3 (PO 4 ) 2 , stirred mechanically at 1500r / min for 120min, filtered the filtrate, added alcohol with a volume fraction of 95% to the filtrate, and kept stirrin...

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Abstract

The invention discloses a method for extracting polysaccharides from the fishbone of hairtail, comprising the following steps: pretreating the fishbone; adding water and alkali into the fishbone so as to perform alkali treatment; adding protease and a zymoexciter to perform enzymolysis; filtering to obtain filter liquor; adding hydrogen peroxide so as to carry out oxidation treatment on the filter liquor; adding Ca3(PO4)2 so as to decontaminate the filter liquor by adsorption; and performing filtering, alcohol precipitation, dehydration and arefaction to prepare the polysaccharides. The method of the invention has the advantages of simple and practical process, easy operation, low energy consumption, less usage of enzyme, short enzymolysis time, saved alcohol usage, lowered production cost, and good quality and high purity of obtained products.

Description

technical field [0001] The invention relates to a method for preparing polysaccharides, in particular to a method for extracting polysaccharides from hairtail fish bones. Background technique [0002] At present, there are many literature reports on the preparation of cartilage polysaccharides from cartilaginous fish such as sharks and stingrays, but there is no research report on the preparation of polysaccharides from hairtail (bony fish) bones. The existing methods for extracting and purifying cartilage polysaccharides are mainly enzymatic hydrolysis, and the steps include: cartilage crushing, lye treatment, enzymolysis and purification. The disadvantages of this method are: slow enzymolysis speed, large enzyme consumption, long reaction time, multiple dissolution and reprecipitation methods, large alcohol consumption, and low product yield. Contents of the invention [0003] The present invention provides a method for extracting polysaccharides from hairtail fish bone...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08B37/00C12S3/02
Inventor 张永勤彭英海申照华郭晓华
Owner SHANDONG MEIJIA GROUP
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