Method for improving growth rate of nostoc flagelliforme in wild culture

A technology for the growth rate of Nostoc hairiformis, applied in the biological field, can solve the problems of few solid culture methods, achieve the effect of improving ecological protection and increasing the growth rate

Inactive Publication Date: 2012-06-27
SHAANXI UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

So far, the liquid culture conditions of Nostocella cells have been relatively mature, but there are still few effective solid culture methods

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0010] Embodiment 1: 1) Preparation of Nostoc hairiform single-cell inoculum: After separation and purification of Nostoc hairiform cells, Nostoc hairiform single-cells were obtained, and the expanded culture conditions of Nostoc hairiform liquid were: light intensity 90 μmolm -2 the s -1 , the light-to-dark ratio is 12:12, the temperature is 25°C when there is light, and 10°C when there is no light, in BG-11 0 Cultivate in the culture medium for 15-20 days to obtain Candida hairy cells, and inoculate the obtained Candida hairy cells into BG-11 with a mass concentration of 0.01% gum arabic 0 In the culture medium, its absorbance A 410 =0.4, that is to make Nostoc hairiform single-cell inoculation bacteria source;

[0011] 2) Cultivation of single cells of Nostoc hairiformis in deserts or deserts in the wild: Spray the inoculum source of single cells of Nostoc hairiformis on deserts or deserts in the wild, spray water once every morning, and the sprayed water makes the surfac...

Embodiment 2

[0012] Embodiment 2: 1) Preparation of Nostoc hairiform single-cell inoculum: After separation and purification of the Nostoc hairiform cells, the Nostoc hairiform single-cell was obtained, and the expanded culture condition of the Nostoc hairiform liquid was 90 μmolm of light intensity -2 the s -1 , the light-to-dark ratio is 12:12, the temperature is 25°C when there is light, and 10°C when there is no light, in BG-11 0 Cultivate in the culture medium for 15-20 days to obtain Candida hairy cells, and inoculate the obtained Candida hairy cells into BG-11 with a mass concentration of 0.3% pectin 0 In the culture medium, its absorbance A 410 =0.6, that is to make Nostoc hairiform single-cell inoculation bacteria source;

[0013] 2) Cultivation of single cells of Nostoc hairiformis in deserts or deserts in the wild: Spray the inoculum source of single cells of Nostoc hairiformis on deserts or deserts in the wild, spray water once every morning, and the sprayed water makes the s...

Embodiment 3

[0014] Embodiment 3: 1) Preparation of Nostoc hairiform single-cell inoculum: After separation and purification of Nostoc hairiform cells, Nostoc hairiform single-cells were obtained, and the conditions for expanding the culture of Nostoc hairiform liquid were: light intensity 90 μmolm -2 the s -1 , the light-to-dark ratio is 12:12, the temperature is 25°C when there is light, and 10°C when there is no light, in BG-11 0 Cultivate in the culture medium for 15-20 days to obtain Candida hairy cells, inoculate the obtained Candida hairy cells into BG-11 containing 0.5% carboxymethyl cellulose 0 In the culture medium, its absorbance A 410 =0.8, that is to make Nostoc hairiform single-cell inoculation bacteria source;

[0015] 2) Cultivation of single cells of Nostoc hairiformis in deserts or deserts in the wild: Spray the inoculum source of single cells of Nostoc hairiformis on deserts or deserts in the wild, spray water once every morning, and the sprayed water makes the surface...

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Abstract

The invention relates to a method for improving the growth rate of nostoc flagelliforme in wild culture. When the nostoc flagelliforme is cultured at a large scale in a wild desert or a harsh desert, the moisture between the nostoc flagelliforme and the surface of a solid attaching substrate is changed and the supply and demand condition of the nostoc flagelliforme for obtaining water from the ambient environment by adding polysaccharide substances (hydrophilic polysaccharide, such as arabicgum, pectin, carboxymethylcellulose, xanthan gum, sodium alginate, and the like), thereby improving thegrowth rate of the nostoc flagelliforme in the wild culture and finally secreting exopolysaccharide by autophage to lead the nostoc flagelliforme and sand grains to bond and form a biological crust and playing an ecological protection role of the nostoc flagelliforme in the control of the harsh desert. The invention is simple and easy and has simple and convenient operation and high cell survivalrate of the nostoc flagelliforme, suitability for large-scale culture, and the like.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a method for increasing the growth rate of Nostoc hairiform in field cultivation, which is suitable for large-scale cultivation of Nostoc hairiform in wild deserts or deserts. Background technique [0002] Nostoc flagelliforme is a terrestrial cyanobacteria (cyanobacteria), commonly known as Nostoc, which mainly grows in some desert steppes and deserts in arid and semi-arid regions. Nostoc hairiform grows very slowly, with an annual growth rate of less than 10% under natural conditions, and its reserves are quite limited. For a long time, Nostoc hairiformis is a kind of rare edible blue-green algae in our country. In recent years, the increase in market demand and price of Nostoc hairiform has intensified the predatory harvesting of Nostoc hairiform, and the resources of Nostoc hairiform have been seriously damaged, so that they are on the verge of depletion, and lead to...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/12C12R1/89
Inventor 陈雪峰王岳尹文翠
Owner SHAANXI UNIV OF SCI & TECH
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