Method for tissue culture and rapid propagation of Trichosanthes kirilowii Maxim

Abstract

The invention relates to the traditional Chinese medicine biotechnological field, in particular to a method for tissue culture and rapid propagation of Trichosanthes kirilowii Maxim, including six steps of configuration of culture medium, picking of explant and establishing of initial generation culture, successive generation propagation culture, strong stock rooting culture, transplantation of seedling in test tube and transplantation to land for growing field crops. By adopting the technical scheme of the invention, (1) as the seedling is completely deviated from the same plant, breed and hereditary characteristics are all the same, thus being convenient for rapid implantation propagation of improved breed; (2) growth of seedling is rapid and tidy and harvest period is almost the same, thus being beneficial to intensive management and commercialization production; (3) plant diseases and insect pests of Trichosanthes kirilowii Maxim are greatly reduced, and cost investment is reduced; (4) by tissue culture and optimization, yield per mu of fresh Trichosanthes kirilowii Maxim fruit can reach more than 5000 kilo grams (increased by 2500-3500 kilo grams), yield of dry Trichosanthes kirilowii Maxim fruits can reach more than 1000 kilo grams (increased by 500-600 kilo grams), and yield of radices trichosanthis can reach more than 1000 kilo grams (increased by 500-600 kilo grams).

Description

technical field [0001] The invention relates to the field of biotechnology of traditional Chinese medicinal materials, in particular to a method for rapid propagation of Trichosanthes chinensis tissue culture. Background technique [0002] At present, the field propagation of Trichosanthes is mostly planted by seeds or tubers. Seed planting, blindness is large, it is difficult to obtain the desired product, and it is easy to cause an imbalance of male and female ratios (Tachia chinensis is dioecious, female plants mainly bear fruits, male plants mainly grow trichosanthes, and in the flowering stage The external forms of the former two are very similar and indistinguishable); root tuber planting is easy to control male and female, but it wastes a lot of trichosanthes medicinal materials that could be used for sale, resulting in a great waste of resources and a sharp decrease in the income of drug farmers, and the speed of reproduction slow. Therefore, we urgently need a new...

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Problems solved by technology

Seed planting, blindness is large, it is difficult to obtain the desired product, and it is easy to cause an imbalance of male and female ratios (Tachia chinensis is dioecious, female plants mainly bear fruits, male plants mainly grow trichosanthes, and in the flowering stage The external forms of the former two are very similar and indistinguishable); root tuber planting is easy to control male and female, but it wastes a lot of trichosanthes medicinal materials that could be used for sale, resulting in a great waste of resources and a sharp decrease in the income of drug farmers, and the speed of reproduction slow

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