Liquid based cell preservative fluid

Abstract

The invention discloses a liquid based cell preservative fluid which is used for matching with an imported or home-made film type liquid based cell film making machine for making films and belongs to the technical field of medicaments. The liquid based cell preservative fluid basically consists of the following components according to the ratios by volume: 10-30 percent of alcohols and the balance of double distilled water; the liquid based cell preservative fluid comprises the following components with the concentration expressed in percentage by mass / volume: 0.1-3.0 percent of phosphate, 0.1-2.0 percent of phenol and 0.1-2.0 percent of N-methyl-N-cyclohexyl-2-amino-3, 5-dibromobenzylamine. Firstly, the invention uses an ordinary biochemical reagent, and the price of consumptive materials is reduced in comparison with the cell preservative fluid produced by an imported auxiliary consumptive material Thinprer TCT. Secondly, because the N-methyl-N-cyclohexyl-2-amino-3, 5-dibromobenzylamine as a unique mucus decomposer is used, the cells wrapped by the mucus in a sample can be successfully separated, avoiding the accumulation and loss of positive cells. Smears prepared by using the liquid based cell preservative fluid to match with an automatic film making machine have clear and transparent backgrounds and uniformly distributed cell monolayers, and are convenient for the film reading of doctors in a pathology department.

Description

technical field [0001] The invention relates to a liquid-based cell preservation solution, which is used in conjunction with imported or domestic membrane-type liquid-based cell production machines. It belongs to the technical field of pharmacy. Background technique [0002] Cervical cancer is one of the most common malignant tumors among female cancers. Early diagnosis of cervical cancer and precancerous lesions is the key to improving the cure rate of cervical cancer and the survival rate of patients. Cytological examination is currently the most widely used clinical method for screening cervical cancer. It is a discipline that diagnoses clinical diseases by observing cell structure and morphological changes. For decades, cytological examination has become the main method for large-scale cervical cancer screening and follow-up observation of high-risk groups, which has greatly reduced the incidence of advanced cervical cancer. [0003] The smear made by the traditional ...

AI Technical Summary

Problems solved by technology

At present, the cell preservation solution (PreserveCyt Solution) produced by Thinprer TCT in the United States is a special reagent matched with its machine Thinprepre-2000. It is expensive, and the cost of one inspection per person is about 180 yuan.

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