Method of adhering loose sands employing microbial mineralization

Abstract

The invention provides a method of adhering loose sands employing microbial mineralization, comprising the following steps: adopting peptone, beef extract and distilled water to prepare culture medium, adjusting pH value to 7.0, sterilizing and drying, inoculating Bacillus pasteurii strain to the prepared culture medium, culturing at 30 DEG C for 24-36h while keeping shaking in the oscillation frequency of 170r / min to ensure that the bacteria solution is completely muddy and the activity k of the bacteria solution is not less than 0.5mmol-min<-1>, mixing urea and calcium chloride solutions ofwhich volumes are equal, and injecting the obtained solution and the bacteria solution separately according the volume ratio of 2:1 in sands or mixing the obtained solution and the bacteria solution and injecting in sands. When the obtained solution and the bacteria solution are separately injected in sands, the bacteria solution is first injected, the mixed solution of urea and calcium chloride is then injected until the bacteria solution leaks out of the sands form top bottom, then the bacteria solution is injected until the solution leaks out and the above steps are repeated. When injectedin sands, the mixed solution is pressed in sands from bottom to top and is discharged from the top of sands. The adhered sand bodies have environmental friend property, simple process and low cost, and the compressive strength can be up to about 2MPa.

Description

technical field [0001] The invention relates to a method for cementing loose grains, in particular to a method for cementing loose sand grains using microbial technology. Background technique [0002] With the continuous destruction of the ecological environment, the degree of desertification is becoming more and more serious; in some areas, due to the high water content of the local soil, it is in a soft state, so in some major construction projects such as the construction of subways, the soil needs to be reinforced; and many cities Built near sandy-based beaches, the entire city is in danger of slipping, caving, sinking. Planting grass and trees is often used in desert prevention and control, and watering cement-based and other cementitious materials are often used in reinforcement measures. However, this type of material is impermeable and seriously damages the ecological performance of the soil, which is not conducive to its sustainable development. Therefore, it is nec...

AI Technical Summary

Problems solved by technology

However, there are still some problems in the use of microbial technology for cementing soil: how to select suitable strains, cultivate and domesticate them; how to improve the weather resistance of microorganisms, etc., and the price of using microbial technology is too expensive

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