Detection method of microfluidic chip based on photoacoustic technique and device thereof

Abstract

The invention relates to a detection method of a microfluidic chip based on a photoacoustic technique and a device thereof. The detection method comprises the following steps of: adopting the microfluidic chip consisting of an incident light generating unit A, a photoacoustic signal detection unit B, a signal processing and data conversion unit C, a computer processing unit D and a platform capable of translating in double directions of X direction and Y direction; adding pretreated samples to be detected into the microfluidic chip for electrophoresis, emitting signals by the computer processing unit according to commands, and controlling electrophoresis and high voltage after being converted by a single chip microprocessor D / A; under the effect of electric field, separating samples with different mobility according to different mobility, absorbing light power by the samples to generate photoacoustic signals by a spot detection window, collecting photoacoustic signals by an ultrasonicsensor, converting the photoacoustic signals into electric signals, converting the photoacoustic signals received by the sensor into digital signals by an analog-to-digital converter and being capable of determining the types and properties of the samples to be detected after being processed by signals of the computer. The detection method has the advantages of high sensitivity, low cost and convenient operation.

Description

A microfluidic chip detection method and device based on photoacoustic technology technical field The invention relates to a detection method and a detection device thereof, in particular to a photoacoustic technology-based microfluidic chip detection method and a device thereof. Background technique Micro Total Analysis System (μ-TAS), also known as Lab-on-a-chip, is a technology that integrates sample preparation, biological and chemical reactions, separation and The detection process is miniaturized or basically miniaturized to a chip of several square centimeters, which is used to complete different biological or chemical reaction processes and analyze its products. It is a micro and comprehensive system. At present, the micro-full analysis system can be divided into two categories: chip type and non-chip type, among which the chip type is the focus of the development of this technology. According to the chip structure and working mechanism of the chip, it can be div...

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Problems solved by technology

CN1410760A and CN2575662Y disclose the microfluidic chip detection system and its device based on the fluorescence detection method, the disadvantage of the fluorescence detection method is that the optical equipment used is complex, bulky, expensive, and the actual price of the fluorescent agent is high

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