Method for simultaneously detecting acetylmethylcar-binol and ligustrazine in vinegar

A technology of acetoin and ligustrazine, which is applied in the field of rapid simultaneous extraction and quantitative detection of acetoin and ligustrazine in vinegar, and achieves the effects of low operator requirements, simple equipment and low detection cost

Inactive Publication Date: 2012-09-19
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The simultaneous detection of acetoin and ligustrazine has not been reported in the literature

Method used

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  • Method for simultaneously detecting acetylmethylcar-binol and ligustrazine in vinegar
  • Method for simultaneously detecting acetylmethylcar-binol and ligustrazine in vinegar
  • Method for simultaneously detecting acetylmethylcar-binol and ligustrazine in vinegar

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0068] Embodiment 1, a kind of method that simultaneously detects acetoin and Ligustrazine in vinegar, comprises the following steps successively:

[0069] 1), pretreatment:

[0070] The vinegar to be tested was pretreated by solvent system-assisted ultrasonic extraction, the volume ratio of extraction solvent to vinegar was 6:1, the extraction solvent was water:glacial acetic acid:ethanol=15:5:80 (volume ratio), and the extraction time was 45min ; Obtained vinegar mixture after pretreatment.

[0071] 2), the vinegar mixture after the above-mentioned pretreatment is detected by the following chromatographic conditions:

[0072] According to the specified method, the detection was performed on an Agilent 1200 liquid chromatograph, using an acid-resistant C18 high-throughput reversed-phase column (100×4.6mm, 1.8 μm), the detection temperature (column temperature) was 45°C, the injection volume was 2 μL, and the detection wavelength was 290nm, the mobile phase is methanol: acid...

Embodiment 2

[0080] Embodiment 2, a kind of method that simultaneously detects acetoin and Ligustrazine in vinegar, comprises the following steps successively:

[0081] 1), pretreatment:

[0082] The vinegar to be tested was pretreated by solvent system-assisted ultrasonic extraction, the volume ratio of extraction solvent to vinegar was 5:1, the extraction solvent was water:glacial acetic acid:ethanol=20:5:75 (volume ratio), and the extraction time was 30min ; Obtained vinegar mixture after pretreatment.

[0083] Step 2) and step 3) are the same as in Example 1.

[0084] Select sample 1~sample 5 in embodiment 1 for use, test result is as shown in table 7 below:

[0085] Table 7 Contents of acetoin and ligustrazine in vinegar

[0086] Sample serial number

Embodiment 3

[0087] Embodiment 3, a kind of method that simultaneously detects acetoin and Ligustrazine in vinegar, comprises the following steps successively:

[0088] 1), pretreatment:

[0089] The vinegar to be tested is pretreated by solvent system-assisted ultrasonic extraction. The volume ratio of the extraction solvent to the vinegar is 7:1, and the extraction solvent is a solution of water:ethanol=1:4 (volume ratio) containing 0.2mol / L of hydrochloric acid. system, the extraction time was 30 minutes; the pretreated vinegar mixture was obtained.

[0090] Step 2) and step 3) are the same as in Example 1.

[0091] Select sample 1~sample 5 in embodiment 1 for use, test result is as shown in table 8 below:

[0092] Table 8 Contents of acetoin and ligustrazine in vinegar

[0093] Sample serial number

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Abstract

The invention discloses a method for simultaneously detecting acetylmethylcar-binol and ligustrazine in table vinegar, which comprises the following steps of: (1) pretreatment: table vinegar to be detected and an extraction solvent are blended, and are extracted by the ultrasound; (2) the pretreated table vinegar mixed liquor is detected according to the following chromatographic condition: the column temperature is 45 DEG C, the sample size is 2 MuL, the detection wavelength is 285 to 300 nm, the mobile phase is the volume ratio of the methanol: water solution containing acid of 55:45; the isocratic elution is carried out; the flow rate is 0.8ml / min; (3) the appearance time of the acetylmethylcar-binol and ligustrazine is 1.278 to 1.299min and 1.982 to 2.039min; according to a chromatogram map, whether the table vinegar containing acetylmethylcar-binol and ligustrazine or not is respectively determined. The method is characterized by the simple and fast operation, good repeatability,high precision, low cost and the like.

Description

technical field [0001] The invention belongs to the field of food detection technology and modern instrument analysis technology, in particular to a method for rapid simultaneous extraction and quantitative detection of acetoin and ligustrazine in vinegar. Background technique [0002] Acetoin is a metabolite of many microorganisms. It is produced by converting diacetyl by α-acetolactate decarboxylase during the brewing process of vinegar. It is an important flavor substance of vinegar, and its content is an important indicator to control the quality of vinegar. Ligustrazine (tetramethylpyrazine) is an important flavor substance in food, and it is also a commonly used food additive, widely present in various foods. At the same time, ligustrazine, as an anti-cardiovascular disease drug, has been clinically used in my country for more than 30 years, and is recognized as safe and has no side effects. Ligustrazine is an alkaloid extracted from the herb Ligusticum chuanxiong. It...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/02G01N30/28G01N30/86
Inventor 何国庆陈继承陈启和卢晓凤吴晶晶阮晖沈生荣
Owner ZHEJIANG UNIV
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