Culture medium for screening enhanced biological phosphorus removal functional bacteria and method for screening enhanced biological phosphorus removal functional bacteria
A technology for biological phosphorus removal and culture medium, which is applied in biochemical equipment and methods, microbial measurement/inspection, and microorganisms, etc. It can solve the problem that the growth of bacteria that is not suitable for enhancing biological phosphorus removal function and the number of bacterial strains that strengthen biological phosphorus removal function are small. , poor targeting and other problems, to achieve the effect of reducing the number of miscellaneous bacteria, the number of miscellaneous bacteria is small, and the nutrients are reasonable
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specific Embodiment approach 1
[0009] Specific embodiment 1: In this embodiment, the medium for screening enhanced biological phosphorus removal functional bacteria is four kinds of mediums used for screening in turn by enhanced biological phosphorus removal functional bacteria, and the four mediums are respectively anaerobic Culture medium, aerobic medium, solid separation medium and liquid separation medium; wherein each liter of anaerobic medium is composed of 0.1-0.3g of NaAC, 130-150mg of (NH 4 ) 2 SO 4 , 13~15mg of CaCl 2 2H 2 O, 170~190mg of MgSO 4 ·7H 2 O, 0.2-0.4mL of trace element solution, 0.8-1.2mL of vitamin solution and the rest of distilled water, the pH value of the anaerobic medium is 7-7.2; each liter of aerobic medium is composed of 130-150mg of KH 2 PO 4 , 230~260mg of K 2 HPO 4 , 120~160mg (NH 4 ) 2 SO 4 , 12~16mg of CaCl 2 2H 2 O. 160-200mg of MgSO 4 ·7H 2 O, 0.1-0.6mL of trace element solution, 0.5-1.5mL of vitamin solution and the rest of distilled water, the pH value ...
specific Embodiment approach 2
[0010] Specific embodiment two: the difference between this embodiment and specific embodiment one is the FeCl in the trace element solution in anaerobic medium, aerobic medium, solid separation medium and liquid separation medium 3 -6H 2 O concentration is 1.5g / L, H 3 BO 3 The concentration is 0.15g / L, CuSO 4 ·5H2 O concentration is 0.03g / L, KI concentration is 0.18g / L, MnCl 2 4H 2 O concentration is 0.12g / L, Na 2 MO 4 2H 2 O concentration is 0.06g / L, ZnSO 4 ·7H 2 The concentration of O is 0.12g / L and CoCl 2 ·6H 2 The concentration of O is 0.15g / L. Others are the same as in the first embodiment.
specific Embodiment approach 3
[0011] Specific embodiment three: what this embodiment is different from specific embodiment one or two is that the vitamin solution in the anaerobic medium, aerobic medium, solid separation medium and liquid separation medium is composed of vitamin B with a concentration of 50mg / L 1 , vitamin B with a concentration of 50mg / L 2 , the concentration is 100mg / L vitamin B 6 , vitamin B at a concentration of 1g / L 12 , folic acid at a concentration of 20mg / L, niacin at a concentration of 50mg / L, calcium pantothenate at a concentration of 50mg / L, p-aminobenzoic acid at a concentration of 50mg / L and biotin at a concentration of 20mg / L. Others are the same as in the second embodiment.
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