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Atap peptides, nucleic acids encoding the same and associated methods of use

A nucleic acid and polynucleotide technology, applied in apoptosis-related proteins, chemical instruments and methods, medical preparations containing active ingredients, etc., can solve problems such as unpleasantness and high toxicity in cancer patients

Inactive Publication Date: 2009-10-14
RUTGERS THE STATE UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0010] Although several current cancer therapies promote cancer cell death and inhibit cancer cell growth, many of these therapies are highly toxic to cancer patients and their administration results in many unpleasant and intolerable side effects
In addition, many currently available cancer therapies have been shown to be effective only for specific etiologies of cancer or hyperplasia

Method used

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  • Atap peptides, nucleic acids encoding the same and associated methods of use
  • Atap peptides, nucleic acids encoding the same and associated methods of use
  • Atap peptides, nucleic acids encoding the same and associated methods of use

Examples

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preparation example Construction

[0165] The production of monoclonal antibodies is well known in the art; see, eg, Harlow et al., Antibodies: A Laboratory Manual, p. 726 (Cold Spring Harbor Pub. 1988). Monoclonal antibodies can be obtained by injecting a mouse or rabbit with a composition comprising the antigen and confirming the presence of the antibody product by removing a serum sample, removing the spleen to obtain B lymphocytes, and fusing the lymphocytes with myeloma cells To generate hybridomas, clone hybridomas, select positive clones that produce antibodies to antigens, and isolate antibodies from hybridoma cultures. Monoclonal antibodies can be isolated and purified from hybridoma cultures by techniques well known in the art.

[0166] In other embodiments, antibodies can be produced recombinantly, eg, by phage display or by combinatorial methods. Phage display and combinatorial methods can be used to isolate recombinant antibodies that bind to ATAP, ATAP-binding proteins, and / or ATAP receptor prote...

Embodiment

[0212] The amphipathic tail anchor domain is conserved in Bfl1 and HCCS1. The gene encoding Bfl1 (BCL2A1 or BCL2-related protein A1) is located on chromosome 15q24.3 and contains three exons, which are transcribed into two alternatively spliced ​​variants, Bfl1(175a.a.) and Bfl1s(163a .a.) (Ko et al., 2003b). The tail anchor (TA) domain of Bfl1 (a.a.147-175) is encoded by exon 3. A database search revealed that exon 3 of BCL2A1 is conserved in HCCS1 (16.4 kb) located on 15q25.1 ( figure 1A). Sequence comparison of the genome sequences of BCL2A1 and HCCS1 revealed that the 8.8 kb fragment of BCL2A1 including exon 3 and the surrounding noncoding regions are highly conserved in HCCS1, which may be due to the duplication of 12 conserved gene segments .

[0213] The genomic sequence of HCCS1 also contains three exons, which encode 91 amino acids ( figure 1 A). The DNA sequence of exon 3 of HCCS1 is 95% identical to that of BCL2A1, and starts at E28 of HCCS1 and E141 of Bfl1 r...

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Abstract

Disclosed herein are nucleic acid sequences that encode pro-apoptotic polypeptides. Also disclosed are polypeptides encoded by these nucleic acid sequences, and antibodies, which immunospecifically-bind to the polypeptide, as well as derivatives, variants, mutants, or fragments of the aforementioned polypeptide, polynucleotide, or antibody. The invention further discloses therapeutic, diagnostic and research methods for diagnosis, treatment, and prevention of proliferative disorders and bacterial infections using the nucleic acids and proteins of the invention.

Description

[0001] References to related applications [0002] Pursuant to 35 U.S.C. §119(e), this application claims priority to U.S. Provisional Application No. 60 / 848,971, filed October 3, 2006, which is hereby incorporated by reference in its entirety for all purposes. [0003] incorporated by reference [0004] According to 37 C.F.R. §1.52(e)(5), the sequence information contained on the file name: ATAP_Ma_2007PCT_SEQList_ST25.txt; size 31KB; created: October 3, 2007; application PatentIn-3.4, and Checker 4.4.0, which The entire contents are incorporated herein by reference. The data in the accompanying filed paper and computer readable sequence listings contain nothing new and are fully supported by priority application US Provisional Patent Application No. 60 / 848,971. [0005] Statement Regarding Federally Funded Research [0006] The US Government has certain rights in this invention due to the following grants: RO1-AG015556, RO1-CA95739, and RO1-HL69000, awarded by the National ...

Claims

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Application Information

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IPC IPC(8): A61K38/00C07K5/00
CPCC07K14/4747C07K2319/21C07K2319/60C07K2319/10A61K48/00A61K38/00A61P31/04A61P35/00A61K38/17C07K14/435C07K19/00
Inventor 麻建杰高载均金哲右诺厄·魏斯勒德
Owner RUTGERS THE STATE UNIV
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