Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Model of outer marrow infiltration of leukemia-NOD/SCID mice and preparing method thereof

A leukemia and acute leukemia technology, which is applied in medical science, material inspection products, surgery, etc., can solve unclear problems and achieve the effect of long survival time and high success rate

Inactive Publication Date: 2009-10-07
天津中医药大学第一附属医院
View PDF0 Cites 5 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is no report on the establishment of human leukemia-NOD / SCID mouse extramedullary infiltration model by tail vein injection of bone marrow mononuclear cells from leukemia patients at home and abroad.
Whether it is possible to establish a long-term surviving human leukemia-NOD / SCID mouse model of extramedullary infiltration by injecting leukemia patients with bone marrow mononuclear cells is still unclear

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Model of outer marrow infiltration of leukemia-NOD/SCID mice and preparing method thereof
  • Model of outer marrow infiltration of leukemia-NOD/SCID mice and preparing method thereof
  • Model of outer marrow infiltration of leukemia-NOD/SCID mice and preparing method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0044] The present invention will be further described in conjunction with the following examples, but the present invention is not limited to the following examples.

[0045] 1. Preparation of bone marrow mononuclear cells from leukemia patients

[0046] Select acute leukemia patients or chronic myelogenous leukemia blast patients, routine aseptic collection of bone marrow 5ml heparin anticoagulant. Using a 15ml centrifuge tube, first inject 5ml of lymphocyte separation solution, and then slowly inject bone marrow fluid to make it float on the upper layer of the separation solution; centrifuge horizontally at 1800r / min for 10min; move the centrifuge tube vertically and slowly into the ultra-clean workbench, and absorb White mononuclear cell suspension layer, washed twice with PBS, counted live cells with 2% trypan blue, and adjusted the final concentration of live cells to 5×10 7 / ml.

[0047] 2. Pretreatment of NOD / SCID mice

[0048] SPF grade NOD / SCID mice, 5 to 6 weeks ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The present invention relates to a method for preparing model of outer marrow infiltration of leukemia-NOD / SCID mice, wherein the method comprises the following steps: 1, preparing a single karyocyte of marrow of leukemia patient, 2. preprocessing the NOD / SCID mice, 3. establishing a model, and 4. identifying the model. The invention firstly adopts a mode of injecting single karyocyte of marrow of leukemia patient through tail-vein injection for establishing the model of outer marrow infiltration of leukemia-NOD / SCID mice. Furthermore the prepared model of outer marrow infiltration of leukemia-NOD / SCID mice has the advantages of high success rate and long survival time. The invention can provide new animal model for researching the outer marrow infiltration mechanism of leukemia, sieving the leukemia medicine, and the targeted therapy of gene and molecule.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a human leukemia-NOD / SCID mouse extramedullary infiltration model and a preparation method thereof. Background technique [0002] With the changes of human living environment, living standards and lifestyles and the advancement of medicine, the spectrum of diseases has undergone significant changes, general infectious diseases have been gradually controlled, and malignant tumors have become increasingly common and seriously threaten human life and quality of life. one of the major diseases. Leukemia is a malignant tumor of the hematopoietic system and one of the most common malignant tumors in my country. According to the survey, the incidence of leukemia in each region occupies the sixth place among various tumors. Leukemia is the malignant proliferation of leukemia cells in bone marrow, spleen, liver and other hematopoietic organs, which can enter the blood circulation and infilt...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): A61B19/00G01N33/48A61B90/00
Inventor 杨文华
Owner 天津中医药大学第一附属医院
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com