Simple method for detecting HLA-G and antibody thereof by gold-labeled immunoassay

Abstract

The invention discloses a simple method for detecting HLA-G and antibody thereof by gold-labeled immunoassay. The invention uses the monoclonal antibody of HLA-G and an HLA-G antigen fragment and / or HLA-G for successfully developing a gold-labeled immunity percolation analysis kit, and a gold-labeled immunochromatography sandwich method and competition method kit, which are used for the public to easily, rapidly and cheaply self-check and self-test the HLA-G in body fluid such as blood, saliva, urine, and the like so as to diagnose the early malignant tumor. In order to diagnose the earlier malignant tumor, a kit for detecting the HLA-G antibody by a gold-labeled immunochromatography indirect method is also developed and used for diagnosing the earlier malignant tumor. The kits are all called as 'broad-spectrum tumor diagnostic kit'. The invention aims at developing an easy, rapid and cheap 'broad-spectrum tumor diagnostic kit' for the self-check and self-test of the public so as to realize primary tumor screening and achieve the goal of finding and curing the tumor early, save the lives of the tumor patients, and reduce the tumor mortality.

Description

Technical field [0001] The invention belongs to the field of biomedical immune analysis. Using gold-label immunoassay technology to detect HLA-G and its antibodies, a new method for simple and rapid diagnosis of malignant tumors has been established for grassroots screening of early malignant tumors. In addition, the present invention can also be used for research in many aspects such as physiology, pathology, pharmacology, immunity, reproduction, tumors, and organ transplantation. technical background [0002] Tumor is a common disease and frequently-occurring disease, ranking second in incidence. According to a 1997 report by the World Health Organization, 6.6 million global cancer patients die each year, and by 2020, the number of cancer patients worldwide will reach 15 million. Various malignant tumors seriously threaten human health. [0003] At present, researchers have discovered a variety of malignant tumor markers, such as AFP (alpha-fetoprotein), CEA (carcinoemb...

AI Technical Summary

Problems solved by technology

However, our country has a vast territory and a large population, and there are certain difficulties in terms of technology, manpower and material resources to carry out basic tumor census according to the current actual situation in our country.

Moreover, the equipment commonly used to detect tumors is relatively complicated at present, and requires special personnel to operate in the laboratory, which is not suitable for grassroots mobile work

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