In vitro application and detection method of peptide for regulating and controlling milk cattle rumen fermentation

A detection method and rumen technology, which are applied in the field of in vitro technology for regulating rumen fermentation of dairy cows, can solve problems such as unclear mechanism of action, and achieve the effects of rapid method, high repeatability, and improved quality of dairy products

Inactive Publication Date: 2012-06-27
NORTHEAST AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the mechanism of action of peptides in the rumen of dairy cows is still unclear.

Method used

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  • In vitro application and detection method of peptide for regulating and controlling milk cattle rumen fermentation
  • In vitro application and detection method of peptide for regulating and controlling milk cattle rumen fermentation
  • In vitro application and detection method of peptide for regulating and controlling milk cattle rumen fermentation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0054] 1. Example 1: Effect of different concentrations of peptides on artificial rumen fermentation parameters

[0055] 1. Test materials

[0056] (1) Artificial rumen simulator

[0057] A medical syringe with an inner diameter of 32mm, a length of 200mm, and a graduated volume of 100ml is used as the fermentor, and a water bath shaker that can accurately control the temperature (±0.5°C) is used as the temperature control device.

[0058] (2) Preparation of artificial rumen buffer (Menke solution)

[0059] The preparation of the buffer was carried out according to the method of Menke et al. (1979). After obtaining the rumen fluid, add the mixed culture solution: 400ml distilled water+0.1ml trace element solution A+200ml buffer solution B+200ml macro element solution C+1ml resazurin solution+40ml reducing agent solution. And mix well, heat to 39℃ while using CO 2 saturation.

[0060] Trace element solution (A): CaCl 2 ·2H 2 O-13.2g, MnCl 2 ·4H 2 O-10.0g, CoCl 2 ·6H 2 O-1.0g, FeCl 3 ·6H...

Embodiment 2

[0117] 2. Example 2: The effect of different concentrations of peptides on artificial rumen enzyme activity

[0118] 1. Test materials

[0119] Same as above

[0120] 2. Experimental design

[0121] Same as above

[0122] The microbial amylase activity, protease activity and carboxymethyl cellulase activity in the fermentation broth in the fermentation tube were measured at each time point.

[0123] 3. Combination of sample determination process and detection method figure 2 .

[0124] According to the conditions and characteristics of rumen microbial fermentation, amylase, protease and carboxymethyl cellulase (using sodium carboxymethyl cellulose as the substrate, mainly endoglucosidase, endo-1,4-β- D-glucanase, referred to as EG) three indicators press figure 2 Carry out the determination of rumen microbial enzyme activity.

[0125] (1) Determination of amylase activity

[0126] The rumen fluid was collected, centrifuged, and the supernatant was taken and measured with an amylase kit...

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Abstract

The invention provides an in-vitro application of peptide which is used for regulating the cow rumen fermentation and a detection method thereof. The method can exactly detect the influence of peptide on the rumen fermentation parameters such as gas production, pH value, ammoniacal nitrogen value, volatile fatty acid and MCP index which are importance indexes for the rumen fermentation. The detailed situation of fermentation in the active rumen can be directly known by the detection; furthermore, the influence of the peptide on the microorganism enzyme activity can be exactly measured; amylase, proteolytic enzyme and carboxymethyl cellulose activity are main enzyme activity indexes in the rumen; the change of microorganism in the active rumen can be directly known by the detection of the three enzymes. The method has the advantages of convenience, being easy for learning, simple and convenient operation, high repeatability, exact numerical value and strong simulation verisimilitude, can effectively determine the approximate consistency which is suitable for peptide in the rumen of cow, and can give out scientific basis and theoretical guidance for deeper theoretic research, preparing the daily ration for the cow more exactly, reducing the waste of protein feeds and improving the quality of the milk products in the future.

Description

(1) Technical field [0001] The invention belongs to the application field of agriculture, animal husbandry and veterinary medicine, and specifically relates to an in vitro technology for regulating and controlling rumen fermentation of dairy cows. (2) Background technology [0002] In recent years, studies have found that small peptide fragments produced by proteolysis have some special physiological effects in addition to nutritional effects. In animal production, it can not only maintain the health of the animal body, but also improve the quality of animal products. However, the mechanism of action of peptides in the rumen of dairy cows is still unclear. (3) Summary of the invention [0003] The purpose of the present invention is to provide an in vitro application and detection method of peptides with the characteristics of fast, repeatable, easy-to-operate, accurate numerical value and strong simulation fidelity for regulating and controlling the fermentation of the rumen of ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/48G01N7/20G01N21/78G01N30/02
Inventor 单安山马宁马清泉
Owner NORTHEAST AGRICULTURAL UNIVERSITY
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