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Method and reagent kit for detecting nitrite shield reagent in milk

A detection kit and nitrite technology, which are applied in the direction of material analysis by observing the influence of chemical indicators, and analysis by chemical reaction of materials, etc., to achieve obvious detection phenomenon, high detection sensitivity, and low detection limit. Effect

Active Publication Date: 2009-04-22
INNER MONGOLIA MENGNIU DAIRY IND (GRP) CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no standard detection method for nitrite shielding agent in milk in the national standard for milk adulteration detection, which cannot meet the actual needs

Method used

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  • Method and reagent kit for detecting nitrite shield reagent in milk
  • Method and reagent kit for detecting nitrite shield reagent in milk
  • Method and reagent kit for detecting nitrite shield reagent in milk

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] This example provides a detection method for nitrite shielding agent in milk. This method requires the addition of nitrite chromogenic agent to detect the content of nitrite in milk. The configuration steps of nitrite chromogenic agent are as follows Describe in detail.

[0027] 1. Configure liquid developer

[0028] In a specific embodiment according to the present invention, liquid chromogen can be used to detect the content of nitrite in milk, and the preparation method of said liquid chromogen is: take 0.1g 1-naphthol, 0.2g 1- Naphthylamine, 0.6g anhydrous p-aminobenzenesulfonic acid, add 200mL distilled water to dissolve, then add 200mL glacial acetic acid after dissolving, and mix well. The chromogenic agent shows different shades of pink in case of different concentrations of nitrite.

[0029] After the liquid nitrite chromogen is prepared, it should be stored at low temperature for future use, for example, it can be stored in a refrigerator.

[0030] 2. Confi...

Embodiment 2

[0034] This example provides a detection method for nitrite shielding agent in milk, and the specific detection steps of the method will be described in detail below. In this embodiment, liquid chromogen and solid chromogen are used to detect the content of nitrite respectively.

[0035] 1. Sample determination:

[0036] (1) Liquid chromogen can be used for detection, the method is as follows:

[0037] Take 1ml of the milk sample to be tested in a test tube, add 0.5ml of 10mg / L sodium nitrite solution, mix well, react for 5-10 seconds, add 0.5ml of liquid color developer, mix well, observe between 0.5-1 minute Color phenomenon.

[0038] When testing each batch of milk samples to be tested, take a blank milk sample without nitrite and nitrite shielding agent at the same time and do a negative blank control experiment in the same way.

[0039] The detection result of the milk sample containing nitrite shielding agent is recorded as positive (+), and the detection result of no...

Embodiment 3

[0069] This example provides a method for detecting a nitrite shielding agent in milk according to another specific embodiment of the present invention, and the specific detection steps of the method will be described in detail below.

[0070] To detect nitrite shielding agent in milk, follow the steps below:

[0071] 1. The preparation method of the nitrite chromogen is the same as in Example 1.

[0072] 2. Sample determination:

[0073] (1) Use liquid color developer to detect, the method is as follows:

[0074] Take 1ml of the milk sample to be tested in a test tube, add 0.5ml of 12mg / L sodium nitrite solution, mix well, react for 5-10 seconds, add 0.5ml of liquid color developer, mix well, observe between 0.5-1 minute Color phenomenon.

[0075] When testing each batch of milk samples to be tested, take a blank milk sample at the same time and do a negative blank control experiment in the same way.

[0076] The detection result of the milk sample containing nitrite shie...

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Abstract

The invention relates to a method used for detecting nitrite screening agent in milk and a kit used for the method. The method comprises the steps as follows: a blank milk sample and a milk sample to be detected are taken, nitrite solution is respectively added and uniformly mixed; the content of the nitrite in the blank milk sample and the milk sample to be detected are respectively detected; if the milk sample to be detected has the same content of the nitrite with the blank milk sample, the milk sample to be detected has no nitrite screening agent; if the detected content of the nitrite in the milk sample to be detected is less than the content of the nitrite in the blank milk sample, the milk sample to be detected has the nitrite screening agent. The method has the advantages of low cost, convenient and quick operation and short detection time; the detection limit is 0.01% and the method of the invention is a quick detection method.

Description

technical field [0001] The invention relates to a method for detecting adulteration in milk and a detection kit thereof, in particular to a method for detecting nitrite shielding agent in milk and a detection kit according to the method. Background technique [0002] The purchased fresh milk may be mixed with some harmful substances due to various reasons. When the content exceeds the specified value, it should be regarded as unqualified milk and disposed of. In addition, there are some cases of adulteration in the purchased fresh milk. For example, adding nitrite to the fresh milk can keep the purchased fresh milk fresh. Usually, the adulterated nitrite content does not exceed 5mg / kg; in order to make the nitrite Nitrate is not detected, and nitrite shielding agents are added to milk, such as sodium ascorbate, ascorbic acid, etc., which will cause nitrite in fresh milk to not be detected normally, which is harmful to adulteration detection and human health. are very unfavo...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/78
Inventor 王丹慧李梅
Owner INNER MONGOLIA MENGNIU DAIRY IND (GRP) CO LTD
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