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Medium test method for extracting oil and protolysate from peanut with water-enzyme method

A technology of hydrolyzing protein and hydrolyzed protein powder, which is applied in the application field of biotechnology in the food industry, and can solve the problems of unseen water enzymatic oil and hydrolyzed protein pilot scale, etc.

Inactive Publication Date: 2012-05-23
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

So far, enzymatic oil extraction has been applied in various oilseeds or oil fruits (peanuts, sunflower seeds, corn germ, etc.) A pilot test has been carried out, and a semi-pilot test has been carried out on coconut, all of which have achieved satisfactory results, but there is no report on the pilot scale of extracting oil and hydrolyzed protein from peanuts by aqueous enzymatic method

Method used

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  • Medium test method for extracting oil and protolysate from peanut with water-enzyme method
  • Medium test method for extracting oil and protolysate from peanut with water-enzyme method

Examples

Experimental program
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Effect test

Embodiment 1

[0034]Dry crush the peeled peanuts with a stone mill, weigh 130kg and add it to an electric heating type 1000L biochemical reactor, then add 650L tap water, mix well, adjust the pH to 9.5 with 2mol / L NaOH, and carry out alkaline extraction at 60°C for 30 minutes; Add Alcalase alkaline protease, the amount of enzyme added is 1.5mL / 100g protein, enzymatically hydrolyze at 60°C for 5h, heat up to 80°C to inactivate the enzyme for 15min; then use a three-phase separator to separate the three phases of the enzymatic hydrolysis system to obtain clear oil I Three parts, oil-water mixture and slag; the obtained oil-water mixture is separated by a disc centrifuge to obtain two parts, one part is oil phase and emulsion, and the other part is oily hydrolyzate. The oil phase and the emulsion are left to stand to obtain clear oil II and the emulsion, wherein the emulsion is demulsified by freezing and thawing to obtain the demulsified oil. The oil-containing hydrolyzate is disc-centrifuged...

Embodiment 2

[0041] Dry crush the peeled peanuts with a stone mill, weigh 150kg and add it to an electric heating 1000L biochemical reactor, then add 750L tap water, mix well, adjust the pH to 8.5 with 2mol / L NaOH, and carry out alkaline extraction at 60°C for 30 minutes; Add Alcalase alkaline protease, the amount of enzyme added is 1.5mL / 100g protein, enzymatically hydrolyze at 60°C for 8h, heat up to 80°C to inactivate the enzyme for 15min; then use a three-phase separator to separate the three phases of the enzymatic hydrolysis system to obtain clear oil I Three parts, oil-water mixture and slag; the obtained oil-water mixture is separated by a disc centrifuge to obtain two parts, one part is oil phase and emulsion, and the other part is oily hydrolyzate. The oil phase and the emulsion are left to stand to obtain clear oil II and the emulsion, wherein the emulsion is demulsified by freezing and thawing to obtain the demulsified oil. The oil-containing hydrolyzate is disc-centrifuged aga...

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Abstract

The invention provides an intermediate test method for extracting oil and hydrolyzed protein from peanut by a water enzyme method, which belongs to the technical field of the application of biological technique in food industry. In the method, the peanut is used as a raw material, single alkali protease is used for enzymolysis, a three-phase separator is used to separate oil, oil-water mixture and insoluble residue simultaneously, a disc-type centrifuge is used to further separate the oil-water mixture so as to obtain emulsion and hydrolysate, wherein the emulsion is subjected to freezing, thawing and emulsion breaking to obtain emulsion breaking oil, and the hydrolysate is subjected to spray drying to obtain peanut hydrolyzed protein powder. The invention provides a better process for industrializing the method of extracting oil and hydrolyzed protein by the water enzyme method, and has the advantages of simple technological line and high utilization rate of products.

Description

technical field [0001] The invention discloses a pilot test method for extracting oil and hydrolyzed protein from peanuts by aqueous enzymatic method, belonging to the technical field of application of biotechnology in food industry. Background technique [0002] Peanut is one of the most important oil crops in the world. The planting area is second only to rapeseed, ranking second in oil crops, and it plays a pivotal role in the world's oil production. my country is one of the important peanut producing countries in the world, with an annual peanut planting area of ​​more than 75 million mu, accounting for more than 20% of the world's peanut planting area, ranking second after India, with an average annual output of more than 14 million tons, accounting for the world's total peanut planting area. More than 42% of the output, ranking first. Peanut oil has a delicate smell, pure taste, rich nutrition, high smoke point (226.7°C), easy to clarify and reuse, and is an excellent ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A23L1/36A23L1/28A23D9/02A23J1/14A23L25/00
Inventor 王璋许时婴张文斌袁博江利华华娣
Owner JIANGNAN UNIV
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