Method for tissue culture and quick propagate technique of reddish blue spider lily

Abstract

The invention discloses a method for the tissue culture of Lycoris haywardii and a rapid propagation technique. The method comprises the following: 1) a step of preparing culture mediums, and components of a basic culture medium and the culture mediums in every stage of tissue culture, as well as the weight of every component contained in each liter are as follows: the basic culture medium comprises MS or 1 / 2MS, wherein the basic culture medium comprises 20 to 40 g / L of sucrose and 8 g / L of agar and has the pH of 5.8; an induction culture medium comprises MS, 0.2 to 1.0 mg / L of TD and 2 g / L of activated carbon; a proliferation culture medium comprises MS, 3 to 7 mg / L of 6-BA and 0.5 to 2.5 mg / L of NAA; a strong seedling culture medium comprises MS, 0.5 to 2.5 mg / L of 6-BA and 0.5 to 1.5 mg / L of NAA; and a rooting culture medium comprises 1 / 2MS, 0.5 to 2.5 mg / L of KT, 0.5 to 2.5 mg / L IBA and 2 g / L of activated carbon; 2) a step of selecting and sterilizing explants; 3) a step of carrying out induction culture, proliferation culture, strong seedling culture and rooting culture; and 4) a step of domesticating and transplanting tissue culture seedlings. The method has the advantages that the induction rate of Lycoris haywardii buds reaches over 90 percent; the proliferation rate of each week is over 500 percent; the growth rate of the Lycoris haywardii buds is accelerated; the browning phenomenon of the explants is effectively prevented; and the rooting of the tissue culture seedlings is promoted at the same time when bulblet browning is prevented.

Description

technical field [0001] The invention relates to the technical field of plant tissue culture, and mainly relates to a method for tissue culture and rapid propagation technology of red and blue Lycoris. Background technique [0002] Lycoris Herb (Lycoris Herb) is a world-recognized famous bulbous flower, known as "Chinese tulip". It is a perennial herbaceous plant with underground bulbs, with rich flower patterns and colors. There are 20 species in this genus worldwide. There are 15 species of Amaryllis in my country, accounting for three-quarters of the global total. [0003] Lycoris haywardii (Lycoris haywardii) is a natural hybrid descendant of the genus Lycoris. It is rarely distributed in nature. Its flowers are lavender rose, and the top of the petals is dark blue, which is very bright in color. The flowering time is from July to September, and the flowering period coincides with the high temperature and few flowers in summer and autumn. It is a rare flower viewing va...

AI Technical Summary

Problems solved by technology

The main reason is that Amaryllis genus plants mainly reproduce by dividing balls naturally. The cue balls with flowering ability only produce 1 to 2 seed balls a year on average, and the reproduction coefficient is quite low; The propagation coefficient through seeds is not high, and it takes 4 to 5 years for seedlings to bloom from development to flowering, which greatly restricts the large-scale development and application of this excellent flower variety.

In recent years, the tissue culture of lycoris has been gradually carried out in China, but it is limited to common species such as lycoris, suddenly laughing, and long-tube lycoris, and no red and blue lycoris have been found that have great potential for development in the field of gardens and fresh cut flowers. Carry out systematic research on tissue culture and rapid propagation technology