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Application of bdellovibrio in removing usual pathogen in fowls egg and meat

A technology of leech vibrio and pathogenic bacteria is applied in the application of common pathogenic bacteria, the elimination of common pathogenic bacteria during storage and processing, and can solve the problems that affect the quality of eggs and meat, the immune effect is not obvious, and the livestock Poultry has problems such as side effects, which can prevent people from suffering from Streptococcus suis, prevent food-borne diseases, and ensure health and safety.

Inactive Publication Date: 2009-01-14
王政军 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] For the control of pathogenic bacteria carried by poultry eggs and meat, there is no information reported at present. The existing information only takes some measures from the process of livestock and poultry breeding, such as: using antibiotics to eliminate or reduce the pathogenic bacteria carried by the living body. Bacteria, although this source measure may have a certain preventive effect, it has side effects on livestock and poultry, directly affecting the quality of poultry eggs and meat
For example, the abuse of antibiotics has caused pathogenic bacteria to develop resistance to antibiotics; although the use of vaccines is a way, the immune effect is not obvious. potential disease risk

Method used

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  • Application of bdellovibrio in removing usual pathogen in fowls egg and meat
  • Application of bdellovibrio in removing usual pathogen in fowls egg and meat

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Application test of Bdellovibrio in eliminating common pathogenic bacteria carried by fresh eggs in poultry farm

[0031] The test includes the following specific steps and conditions:

[0032] Step 1: Separation and purification of Bdellovibrio

[0033]Pre-treat the collected water body or sediment, inoculate the Aeromonas hydrophila proliferating Bdellovibrio, invert the double-layer plate, and plaques appear on the double-layer plate containing the host bacteria, and pick the expanding single plaque , Then according to the microbiological method, liquid proliferation culture, double-layer plate test, pick the expanding single plaque again, and repeat it several times, until the single plaque is passaged to form a plaque with the same shape, size, and transparency, that is, According to the size of a single spot in the same time, two pure strains of Budovibrus were isolated from a pure strain of Budovibrus. The Budovibrus was found on January 13, 2008 in Wuhan University,...

Embodiment 2

[0044] Application Test of Budovibrio in Eliminating Common Pathogenic Bacteria Carried by Livestock Meat

[0045] The test includes the following specific steps and conditions:

[0046] Steps 1, 2 and 3 (1) are the same as in Example 1

[0047] Step three (2) 2 strains of Budovibrio Budovibrus to eliminate Salmonella, Streptococcus suis, Clostridium and Escherichia coli carried on the surface of pork during processing

[0048] Separate each piece of experimental sample pigs with similar weights, each weighing about 0.25kg. The experiment has a control group and an experimental group. The control group has three pieces, which are three parallel samples, and the remaining pork pieces are the experimental group. Each group has three pieces. Block, for three parallel samples. Soak them in the pathogenic bacteria mixture of step 3 (1). The pathogenic bacteria mixture refers to the final concentration of Salmonella, Streptococcus suis, Clostridium and Escherichia coli respectively reac...

Embodiment 3

[0060] Application test of Bdellovibrio in eliminating common pathogenic bacteria in pig farm environment

[0061] The test includes the following specific steps and conditions:

[0062] Steps 1 and 2 are the same as in Example 1

[0063] Step three (1) Elimination test of pathogenic bacteria

[0064] Take a pig farm as an example. One year before the application of Budovibrosis, due to poor hygienic conditions of pig breeding, there were more Streptococcus suis and Escherichia coli in the breeding environment, often reaching 10%. 4 cfu / cm 2 the above. Therefore, in order to control their diseases, the farms often add antibiotics to the feed, which causes the quality of pork to decline and export trade is restricted. At the same time, the breeding sanitary conditions are not good, and the pigs are prone to enteritis, swine pneumonia and other diseases.

[0065] In order to explore a healthy and green pig raising method, the pig farm tried the application method of the present inve...

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Abstract

The invention discloses the application of bdellovibrio in removing the commonly seen pathogen in poultry, eggs and meat; the poultry, eggs and meat with pathogen are treated by bdellovibrio liquid in a spraying, rubbing or soaking way; when the soaking method is adopted, the concentration of the bdellovibrio at least reaches 10<2>puf / ml; when the spraying or the rubbing way is adopted and the use of bdellovibrio liquid with concentration equal to or more than 10<2>pfu / ml, the consumption at least reaches 15ml / kg. The disclosed two units of bdellovibrio can be used independently, and the effect after the two units are mixed is better. The application of the invention is convenient in use, safe, has no side effect, obvious effect in removing commonly seen pathogen in poultry, eggs and meat, germ clearing rate of more than 90 percent, is applicable to the whole process from cultivation to dining including poultry, egg and meat processing, directing improves the product quality, and very significant improving food safety and ensuring the health of human beings.

Description

Technical field [0001] The invention belongs to the field of biotechnology, relates to bacteria and their applications, and specifically refers to the application of Budovibrio in eliminating common pathogenic bacteria carried by poultry eggs and meat, and is particularly suitable for livestock and poultry breeding environments and the preservation of poultry eggs and meat And the elimination of common pathogenic bacteria during processing. Background technique [0002] Food safety has become a common concern all over the world. The World Health Organization (WHO) regards food safety as a worldwide challenge. In recent years, global food safety incidents have occurred frequently, and food-borne diseases have become an important issue affecting public health. According to WHO statistics, about one-third of people in developed countries are infected with foodborne diseases each year, and in some developing countries, foodborne diseases are often the main cause of abnormal deaths. T...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23B5/16A01N63/00A01P1/00C12N1/20
Inventor 蔡俊鹏马小花
Owner 王政军
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