Application of bdellovibrio in removing usual pathogen in fowls egg and meat
A technology of leech vibrio and pathogenic bacteria is applied in the application of common pathogenic bacteria, the elimination of common pathogenic bacteria during storage and processing, and can solve the problems that affect the quality of eggs and meat, the immune effect is not obvious, and the livestock Poultry has problems such as side effects, which can prevent people from suffering from Streptococcus suis, prevent food-borne diseases, and ensure health and safety.
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Embodiment 1
[0030] Application test of Bdellovibrio in eliminating common pathogenic bacteria carried by fresh eggs in poultry farm
[0031] The test includes the following specific steps and conditions:
[0032] Step 1: Separation and purification of Bdellovibrio
[0033]Pre-treat the collected water body or sediment, inoculate the Aeromonas hydrophila proliferating Bdellovibrio, invert the double-layer plate, and plaques appear on the double-layer plate containing the host bacteria, and pick the expanding single plaque , Then according to the microbiological method, liquid proliferation culture, double-layer plate test, pick the expanding single plaque again, and repeat it several times, until the single plaque is passaged to form a plaque with the same shape, size, and transparency, that is, According to the size of a single spot in the same time, two pure strains of Budovibrus were isolated from a pure strain of Budovibrus. The Budovibrus was found on January 13, 2008 in Wuhan University,...
Embodiment 2
[0044] Application Test of Budovibrio in Eliminating Common Pathogenic Bacteria Carried by Livestock Meat
[0045] The test includes the following specific steps and conditions:
[0046] Steps 1, 2 and 3 (1) are the same as in Example 1
[0047] Step three (2) 2 strains of Budovibrio Budovibrus to eliminate Salmonella, Streptococcus suis, Clostridium and Escherichia coli carried on the surface of pork during processing
[0048] Separate each piece of experimental sample pigs with similar weights, each weighing about 0.25kg. The experiment has a control group and an experimental group. The control group has three pieces, which are three parallel samples, and the remaining pork pieces are the experimental group. Each group has three pieces. Block, for three parallel samples. Soak them in the pathogenic bacteria mixture of step 3 (1). The pathogenic bacteria mixture refers to the final concentration of Salmonella, Streptococcus suis, Clostridium and Escherichia coli respectively reac...
Embodiment 3
[0060] Application test of Bdellovibrio in eliminating common pathogenic bacteria in pig farm environment
[0061] The test includes the following specific steps and conditions:
[0062] Steps 1 and 2 are the same as in Example 1
[0063] Step three (1) Elimination test of pathogenic bacteria
[0064] Take a pig farm as an example. One year before the application of Budovibrosis, due to poor hygienic conditions of pig breeding, there were more Streptococcus suis and Escherichia coli in the breeding environment, often reaching 10%. 4 cfu / cm 2 the above. Therefore, in order to control their diseases, the farms often add antibiotics to the feed, which causes the quality of pork to decline and export trade is restricted. At the same time, the breeding sanitary conditions are not good, and the pigs are prone to enteritis, swine pneumonia and other diseases.
[0065] In order to explore a healthy and green pig raising method, the pig farm tried the application method of the present inve...
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