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Anti-cataract target protein

A cataract and target protein technology, which is applied in the fields of medicine, food and beverage, can solve the problems of poor pertinence, no report on the function of DTT crystal protein, and large side effects

Inactive Publication Date: 2009-01-07
SECOND MILITARY MEDICAL UNIV OF THE PEOPLES LIBERATION ARMY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0048] There are many drugs currently used to treat cataract in clinical practice. Due to the lack of perfect basis for the formation mechanism of cataract, many drugs that are still widely used have many defects: some have low efficiency, poor pertinence, and some have relatively large side effects.
[0057] According to literature search, so far, no new and better target protein has been found based on the mechanism of cataract formation, and no research report on the function of DTT on crystal protein has been found. Reports on targets, active ingredients or products with good application effects for prevention, diagnosis, detection, protection, treatment and research of cataract and related diseases

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0357] Embodiment 1, heat-induced denaturation experiment

[0358] The present invention uses heat-induced denaturation experiments to analyze βB 2 Biochemical properties of βB; 2 Sensitivity to heat denaturation, βB 2 For other proteins affected by thermal aggregation and denaturation (analogous to the molecular chaperone effect of α family crystal proteins) and the special compound dithiothreitol for βB 2 Protection against thermal denaturation of crystal proteins.

[0359] (1) Materials and methods

[0360] 1. Reagents: Alcohol dehydrogenase (abbreviation: ADH), DTT were purchased from Sigama Company; βB 2 The crystal protein was expressed and purified by our laboratory.

[0361] 2. Instruments: Bio-Rad Smartspec3000 photometer; DK-60 electric heating constant temperature water tank; 1.0ml quartz cuvette (the lower part is a slit cell, 0.5ml can be used for colorimetric detection).

[0362] 3. Protein solution preparation:

[0363] (1) Preparation of 0.4mg / ml ADH, DT...

Embodiment 2

[0397] Example 2, one of the product formulations designed for the target protein

[0398] Taurine 11mmol / L (ie: 1376mg / L)

[0399] Lysine 12mmol / L (ie: 1752mg / L)

[0400] Dithiothreitol 2.5mmol / L (ie: 385mg / L);

[0401] The composition is composed of taurine, free lysine and dithiothreitol. If it is made into eye drops for external use, it is 1-3 times a day, 1 drop each time.

Embodiment 3

[0402] Example 3, the second product formula designed for the target protein

[0403] Taurine 111mmol / L (ie: 13765mg / L)

[0404] Lysine 120mmol / L (ie: 17520mg / L)

[0405] Dithiothreitol 0.25mmol / L (ie: 38mg / L);

[0406] The composition is composed of taurine, free lysine and dithiothreitol. If it is made into eye drops for external use, it is 1-3 times a day, 1 drop each time.

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Abstract

The invention relates to a target protein for resisting cataract and the application thereof. The change rule of a crystallin beta B2 with special expression and special distribution along with the body aging process is discovered according to the phase change of the crystallin, and the crystallin is determined as the more appropriate target protein for resisting cataract. Also discovered is that dithiothreitol is the substance capable of effectively inhibiting denaturation by heat of crystallins such as beta B2. Accordingly, corresponding anti-cataract products can be prepared by selecting the target protein as the target point and using dithiothreitol and composition thereof as the raw materials. Cataract disease is an important pathogenic reason influencing the quality of life of Chinese people. The beta B2 is determined as the target protein with wide application, and provides the direction for the development of new drugs. The developed product is mainly used for prevention, and is used externally in combination. A novel drug resource is provided, and is the effective product for preventing, diagnosing and treating denaturation of crystallin or cataract and other related diseases, thereby generating remarkable social and economic benefits.

Description

technical field [0001] The present invention relates to the technical fields of medicine, food and beverage, in particular to a target protein which can be used to prepare related products and its use, more specifically to a target protein which can be used to resist cataract and its use . Background technique [0002] (1) Overview of cataract research [0003] 1 Overview [0004] Cataract is one of the most common causes of blindness, and is a common and frequently-occurring disease that seriously affects human health. Clinical studies have shown that among various cataract cases, senile cataracts caused by aging account for the vast majority. [0005] Senile cataract is a binocular disease, but the onset of the two eyes may occur successively. According to the location where the cataract begins to form, senile cataract is divided into cortical cataract, nuclear cataract and subcapsular cataract, among which cortical cataract is the most common type of senile cataract. ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/47A61K31/385A61K31/095A61K38/06A61K31/616A61K31/197A61K31/185A61P27/12G01N33/68A23L1/30A23L33/125A23L33/17
Inventor 李闻捷张军刘梦蕾
Owner SECOND MILITARY MEDICAL UNIV OF THE PEOPLES LIBERATION ARMY
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