Eureka AIR delivers breakthrough ideas for toughest innovation challenges, trusted by R&D personnel around the world.

Expression and applications of novel non-restriction endonuclease in escherichia coli

An endonuclease, non-restrictive technology, applied in the field of non-specific endonuclease, can solve the problems of high price, cumbersome process, low yield, etc.

Inactive Publication Date: 2008-11-26
SHANGHAI BAILANG BIOTECHOLOGY
View PDF0 Cites 6 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, the yield of this enzyme is low, the process is cumbersome, and the price is relatively expensive, which is not suitable for use in my country.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Expression and applications of novel non-restriction endonuclease in escherichia coli
  • Expression and applications of novel non-restriction endonuclease in escherichia coli
  • Expression and applications of novel non-restriction endonuclease in escherichia coli

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

Embodiment 2

Embodiment 3

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention utilizes the property that nonspecific endonuclease from Anabaena sp. is provided with natural inhibitor protein to change the original expression way of the nonspecific endonuclease, i.e. to change secretory expression into endoenzyme high efficient expression. The invention adopts a strong promoter to largely express in cells in a way of inclusion bodies with no activity; wherein, the moment when the endonuclease is expressed, the natural inhibitor protein is expressed together so as to eliminate the strong toxicity that farthing activated nuclease imposes on the cells; protein engineering technology is used for replacing a non-activated central amino acid, thus reducing the possibility of forming multimer in renaturation, improving efficiency, and furthermore, raising the output in terms of improving the expression efficiency and the renaturation efficiency.

Description

technical field The invention relates to a method for producing a non-specific endonuclease by recombinant DNA technology. The enzyme can degrade various forms of nucleic acids (including DNA and RNA), and can be used to remove nucleic acids in biological materials containing nucleic acids as impurities or eliminate the influence caused by nucleic acids. Background technique Non-specific endonucleases are a class of hydrolytic enzymes that can degrade various forms of DNA and RNA, and have high phosphodiester bonds for single-stranded, double-stranded, linear, circular and supercoiled forms of DNA and RNA. activity to produce 5'-phosphate nucleotides or 5'-phosphate oligonucleotides, and has no sequence requirement for nucleic acids. It can be used to remove nucleic acids in biological materials or to exclude interference caused by nucleic acids. A typical representative of this type of enzyme is a non-specific endonuclease derived from Serratia marcescens, which has been...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/55C12N9/22C12N1/21C12P19/30C12Q1/68
Inventor 汪寄宇张建新
Owner SHANGHAI BAILANG BIOTECHOLOGY
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Eureka Blog
Learn More
PatSnap group products