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Polypeptides with donkey hide gelatin and method for preparing same

A technology of donkey-hide gelatin and compound enzymes, which is applied in the direction of specific peptides, medical formulas, connective tissue peptides, etc., can solve the problems of unsatisfactory absorption effect, low donkey-hide gelatin proportion, and large molecular weight of donkey-hide gelatin, so as to achieve energy saving and consumption reduction. Production efficiency, the effect of simplifying the operation process

Active Publication Date: 2008-10-15
北京华达杰瑞生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Traditional donkey-hide gelatin blocks are very inconvenient to take, requiring long-term stewing and soaking operations
In order to improve the convenience of taking donkey-hide gelatin, a lot of improvements have been made to the dosage forms of donkey-hide gelatin, such as instant granules, donkey-hide gelatin pulp, etc. The disadvantage is that the proportion of donkey-hide gelatin in it is relatively low
Although nano-gelatin can make up for this shortcoming and improve the solubility, due to the large molecular weight of donkey-hide gelatin, it is still impossible to obtain a uniform and stable solution after being dissolved in water.
Moreover, due to the large molecular weight, the absorption effect of the human body is not ideal.
The concept of small molecule donkey-hide gelatin was mentioned in the patent introduction of donkey-hide gelatin phospholipid composition in 2004, and the patent literature of donkey-hide gelatin polypeptide wine appeared in 2007, but these two products appeared in the form of compound, which was not conducive to the production of donkey-hide gelatin polypeptide. Used as a separate component in other formulations or dosage forms

Method used

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  • Polypeptides with donkey hide gelatin and method for preparing same
  • Polypeptides with donkey hide gelatin and method for preparing same
  • Polypeptides with donkey hide gelatin and method for preparing same

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0056] Compound enzyme preparation: Weigh 10g of neutral protease, 15g of papain, 50g of pineapple enzyme, 5g of alkaline protease, and 20g of flavor enzyme and mix them uniformly to obtain 100g of compound enzyme for use.

[0057] Add 15 grams of pulverized donkey-hide gelatin granules and 85ml of water to make an aqueous solution, heat up to 55°C to dissolve, adjust the pH of the system to 5.5-6.0 with citric acid buffer solution, add 0.15g of compound enzyme to react for 3 hours, and heat to above 85°C Keep it for 15 minutes, filter the enzymatic solution, concentrate, and spray-dry to obtain dry donkey-hide gelatin polypeptide powder. The amino acid and trace element components, solubility, viscosity, molecular weight and distribution of the product are measured and compared with traditional donkey-hide gelatin. The results are shown in Table 1.

Embodiment 2

[0059] Preparation of compound enzyme: Weigh 15g of papain enzyme, 55g of pineapple enzyme and 30g of flavor enzyme respectively and mix them uniformly to obtain 100g of compound enzyme for use.

[0060] Add 15 grams of pulverized donkey-hide gelatin granules and 85ml of water to make an aqueous solution, heat up to 60°C to dissolve, adjust the pH of the system to 5.5-6.0 with citric acid buffer solution, add 0.12g of compound enzyme to react for 3 hours, and heat to above 85°C Keep it for 15 minutes, filter the enzymatic solution, concentrate, and spray-dry to obtain dry donkey-hide gelatin polypeptide powder. The amino acid and trace element components, solubility, viscosity, molecular weight and distribution of the product are measured and compared with traditional donkey-hide gelatin. The results are shown in Table 1.

Embodiment 3

[0062] Preparation of compound enzyme: Weigh 20 g of neutral protease, 20 g of papain, 40 g of pineapple enzyme, and 20 g of flavor enzyme and mix them uniformly to obtain 100 g of compound enzyme for use.

[0063] Add 15 grams of pulverized donkey-hide gelatin granules and 85ml of water to make an aqueous solution, heat up to 60°C to dissolve, adjust the pH of the system to 5.5-6.0 with citric acid buffer solution, add 0.075g of compound enzyme to react for 3 hours, and heat to above 85°C Keep it for 15 minutes, filter the enzymatic solution, concentrate, and spray-dry to obtain dry donkey-hide gelatin polypeptide powder. The amino acid and trace element components, solubility, viscosity, molecular weight and distribution of the product are measured and compared with traditional donkey-hide gelatin. The results are shown in Table 1.

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Abstract

The invention provides donkey-hide gelatin polypeptide and a method for preparing the same. The method comprises the following steps that donkey-hide gelatin is reacted with biologic compound enzyme under certain conditions, and the reacted compound is filtered and dried, etc., to obtain the donkey-hide gelatin polypeptide. The method has simple steps, easy operation and control, and low cost. Moreover, the donkey-hide gelatin polypeptide produced according to the method has steady property and easy preservation, can be dissolved into cold water to form steady solution, and can be taken as an independent composition to directly be applied to compound products.

Description

technical field [0001] The invention relates to a new hydrolyzed donkey-hide gelatin polypeptide and a preparation method thereof. Background technique [0002] As a traditional Chinese medicine, donkey-hide gelatin is actually a biological product, and it is also a health care medicine. Traditional donkey-hide gelatin cubes are very inconvenient to take, requiring long-time stewing and soaking operations. In order to improve the convenience of taking donkey-hide gelatin, a lot of improvements have been made to the dosage forms of donkey-hide gelatin, such as instant granules, donkey-hide gelatin paste, etc. The disadvantage is that the proportion of donkey-hide gelatin in it is relatively low. Although nano donkey-hide gelatin can make up for this shortcoming and improve the solubility, due to the large molecular weight of donkey-hide gelatin, it is still impossible to obtain a uniform and stable solution after the nano-donkey-hide gelatin preparation is dissolved in water...

Claims

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Application Information

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IPC IPC(8): C12P21/06C07K14/78A61K35/36
Inventor 邓洁丽蒋国昌
Owner 北京华达杰瑞生物技术有限公司
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