Polymorphism mark screening of chlamys ferrari G-type lysozyme gene and auxiliary breeding means

Abstract

The invention relates to a method for screening of chlamys farreri G-type muramidase gene polymorphism markers and the assistant breeding method thereof, belonging to shellfish molecular marker assistant breeding technology in the filed of aquatic organism technology, mainly comprising the steps of: cloning partial sequence of promoter region of a chlamys farreri G-type muramidase gene, preparing disease-resistance population and sensitive population, screening of disease-resistance G-type muramidase gene markers, quickly screening the disease-resistance population and building gene marker assistant breeding technology. According to the invention, promoter region sequence of the chlamys farreri G-type muramidase gene is cloned, and its polymorphism sites are screened. The occurrence frequency of -391 AG individual in disease-resistance population is obviously higher than that in sensitive population, therefore, resistance-related gene marker assistance breeding method is built with -391 AG as chlamys farreri resistance-related G-type muramidase gene marker. The invention has the characteristics of strong pertinence, high breeding efficiency, simple and quick operation, etc., and is suitable for screening of shellfish resistance-related markers and breeding of disease-resistance fine varieties.

Description

Technical field: [0001] The invention belongs to the shellfish molecular marker assisted breeding technology in the technical field of aquatic organisms, and relates to a shellfish disease resistance-related gene marker and an assisted breeding method that can be used in the cultivation of shellfish disease-resistant varieties. Background technique: [0002] After the vigorous development of the scallop aquaculture industry in our country, it has gradually exposed many problems that need to be solved urgently. Long-term inbreeding and multigenerational breeding lead to inbreeding depression, which makes Chlamys farreri grow slowly and reduce stress resistance. Coupled with factors such as raging diseases and environmental deterioration, large-scale deaths often occur in the scallop farming industry, causing huge economic losses. Therefore, accelerating the research on core cutting-edge technologies of breeding and the selection of excellent stress-resistant varieties has be...

AI Technical Summary

Problems solved by technology

Current studies have shown that the increase in the expression level of lysozyme in various species such as fish and insects is closely related to the enhancement of immunity (Caipang CM, 2007; Cheng AC, 2008), but its polymorphism is associated with shellfish disease resistance. Research on the relationship between strength and weakness has not yet been reported

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