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Molecule marker of brassica napus self-incompatible maintainer as well as preparation and uses thereof

A Brassica napus and molecular marker technology, which is applied in the directions of biochemical equipment and methods, DNA preparation, and microbial determination/inspection, etc., can solve the problem of molecular markers and preparation methods of Brassica napus self-incompatibility maintainers that have not yet been seen. application and other issues to achieve the effect of reducing the workload of breeding, speeding up the pace, and shortening the breeding years

Inactive Publication Date: 2008-08-27
HUAZHONG AGRI UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] So far, there have been no reports on molecular markers, preparation methods and applications of self-incompatibility maintainer lines in Brassica napus

Method used

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  • Molecule marker of brassica napus self-incompatible maintainer as well as preparation and uses thereof
  • Molecule marker of brassica napus self-incompatible maintainer as well as preparation and uses thereof
  • Molecule marker of brassica napus self-incompatible maintainer as well as preparation and uses thereof

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Experimental program
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Effect test

Embodiment 1

[0038] 1. Production of Brassica napus self-incompatible hybrid F 1

[0039] Brassica napus self-incompatibility line S-1300 (see literature: Ma Chaozhi et al., Breeding of Brassica napus double-low self-incompatibility line, Journal of Huazhong Agricultural University, 1998, 17(3): 211-213 ) as the female parent, and Brassica napus self-incompatibility maintainer line 04P63 wai-52 (this line was preserved in the Chinese Type Culture Collection Center (English abbreviation: CCTCC) of Wuhan University, Wuhan City, Hubei Province on September 20, 2007 ), the preservation number is: CCTCC NO: P200704, the classification of this variety is named: Brassica napus) is the male parent for crossing, and obtains F 1 seed.

[0040] 2. Screening self-incompatibility segregation populations of Brassica napus

[0041] F obtained by the above steps 1 Seeds were sown under natural conditions to obtain Brassica napus self-incompatibility F 1 plants. in F 1 (S-1300×04P63external-52)F 2 ...

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Abstract

The invention belongs to the rapeseed breeding technical field and in particular relates to a dominant SCAR molecular marker of a brassica napus self-incompatible maintainer line, which is characterized in that a reported primer SP11a is utilized to amplify genome DNA of a brassica napus self-incompatible line S-1300 and the brassica napus self-incompatible maintainer line, amplified DNA fragments are respectively obtained, then, the amplified DNA fragments are cloned and sequenced to compare nucleotide sequences, a primer SP11b is designed according to the difference of the sequences, PCR is amplified, the dominant SCAR molecular marker of the brassica napus self-incompatible maintainer line is obtained, and the nucleotide sequence of the dominant SCAR molecular marker is displayed in a sequence table SEQ ID NO:3. The invention also discloses a method for preparing the molecular marker and application of the molecular marker.

Description

technical field [0001] The invention belongs to the technical field of rapeseed breeding, and specifically relates to a molecular marker for creating a self-incompatibility maintenance line of Brassica napus, a preparation method and an application. Background technique [0002] Self-incompatibility is an important way to utilize heterosis in Brassica napus. Compared with cytoplasmic male sterility, which is the current main way of utilizing heterosis in Brassica napus, self-incompatible hybrids have the advantages of short breeding cycle, many restorer lines, no adverse cytoplasmic effects, and high seed production yield. It is one of the hotspots of research by breeders at home and abroad. Self-incompatibility of self-incompatibility materials cannot produce fruit. At present, there is no method for mass propagation of self-incompatibility lines, which limits the production and application of self-incompatibility hybrids. At present, the methods of breeding self-incompat...

Claims

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Application Information

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IPC IPC(8): C12N15/11C12N15/10C12Q1/68
Inventor 马朝芝唐家友张幸果高长斌唐维
Owner HUAZHONG AGRI UNIV
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