Method for improving construction success ratio of agnate half-sib family by breeding turbot family

Abstract

The invention provides a method for improving the success rate of establishing father half- sib family through the selective breeding of turbot family and comprises the steps that first, parental turbots used for family selective breeding are cultured as parental fish and marked individually; second, the sex gland of the parental turbot can achieve synchronous maturity by reproductive control; third, the parental fish, the sex gland development of which is in the peak stage, is selected for artificial insemination; then the father half-sib family is established according to the method that one male fish is mated with 2-3 pieces of female fish. The establishment of the father half-sib family is carried out again if the initial establishment fails. The invention applied to the selective breeding of the turbot family has simple operation and remarkable effect and the success rate of the initial establishment of the father half-sib family can be remarkably improved to more than 33.3 percent; the established father half-sib families have good synchronism, which dramatically shortens the family establishment period and is beneficial for the standard culture of the turbot family.

Description

Technical field: [0001] The invention belongs to the technology of turbot family selection and breeding, and is a method for improving the success rate of paternal half-sib family construction. Background technique: [0002] Internationally, large-scale family selection techniques have been used to genetically improve aquaculture animals, and significant results have been achieved, such as the genetic improvement of Atlantic salmon in Norcheng (Gjerde et al, 1999), and the selection of Indian carp (Basanta et al, 2000), the selection of tilapia in the Philippines (Gall et al, 2002), the selection of rainbow trout in the United States (Ronald et al, 1993) and the selection of resistance to marine shrimp in Colombia (Amezquita et al, 1997), all achieved good breeding results; while domestic research on aquatic animals is still in its infancy, and domestic turbot breeding has just begun. The establishment of male half-sib families is of great significance in the estimation of ...

AI Technical Summary

Problems solved by technology

[0002] Internationally, large-scale family selection techniques have been used to genetically improve aquaculture animals, and significant results have been achieved, such as the genetic improvement of Atlantic salmon in Norcheng (Gjerde et al, 1999), and the selection of Indian carp (Basanta et al, 2000), the selection of tilapia in the Philippines (Gall et al, 2002), the selection of rainbow trout in the United States (Ronald et al, 1993) and the selection of resistance to marine shrimp in Colombia (Amezquita et al, 1997), all achieved good breeding results; while domestic research on aquatic animals is still in its infancy, and domestic turbot breeding has just begun. The establishment of male half-sib families is of great significance in the estimation of genetic parameters and the formulation of breeding programs. Due to factors such as the number and quality of eggs laid in the second laying are unstable, the amount of semen of male fish is small and unstable, and the survival rate of seedlings is unstable, there are problems such as the low success rate of the initial construction of the paternal half-sib family and the asynchronous construction of the paternal half-sib family