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Tea geometrid acetaldehyde dehydrogenase gene adh and application thereof

A kind of technology of acetaldehyde dehydrogenase and tea looper, applied in the field of insect biology, can solve the problems of unexplained death, poor work efficiency, time-consuming and labor-intensive problems

Inactive Publication Date: 2011-09-28
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Capture directly from nature, time-consuming and labor-intensive, poor work efficiency
The artificial feeding method can rapidly expand the insect population, but unexplained deaths often occur during the insect breeding process, which brings losses to the insect breeding

Method used

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  • Tea geometrid acetaldehyde dehydrogenase gene adh and application thereof
  • Tea geometrid acetaldehyde dehydrogenase gene adh and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Ammonia, industrial alcohol and water are blended in different proportions into three types of environmental stress fumigants: the low-dose fumigant is an aqueous solution containing 0.01-0.5% ammonia and 0.1-0.5% alcohol by volume, and the medium-dose fumigant is an aqueous solution containing 0.6- Aqueous solution of 1.5% ammonia water and 0.5-1% alcohol, high-dose fumigant liquid is an aqueous solution containing 1.5-6% ammonia water and 1.1-5% alcohol. Use adhesive tape to fix a group of absorbent cotton balls in the center of 4 petri dishes with a diameter of 21cm. 2-10ml, the same volume of pure water was added dropwise as a control. Forty third-instar tea geometrid larvae reared were divided into 4 groups on average, placed in 4 petri dishes respectively, and after adding appropriate amount of fresh tea leaf food, the petri dish was covered. Tea geometrids after fumigation for 6 hours were used for cDNA-AFLP analysis. For each treatment, 2-3 larvae were placed ...

Embodiment 2

[0028] Fix a group of absorbent cotton balls with adhesive tape in the center of 4 petri dishes with a diameter of 21 cm, and drop 2ml or 20ml of water on the cotton balls to simulate humidity stress, of which 2ml is the normal humidity group and 20ml is the high humidity stress group. 200 2nd instar tea geometrid larvae were divided into two groups, one group was fed under normal humidity conditions, and the other group was fed under high humidity conditions. There are 2 dishes in each group, one dish is used for mortality observation, and the other dish is used for gene expression research. After culturing for 12 and 72 hours, 2-4 surviving larvae were selected from the normal humidity feeding group and the high humidity feeding group respectively. Total RNA was extracted by TRIZOL (Invitrogen Company), and cDNA was synthesized by MMLV first-strand cDNA synthesis kit (Shanghai Sangon Bioengineering Co., Ltd.). PCR and electrophoresis were performed using the primer combinat...

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Abstract

The present invention discloses an aldehyde dehydrogenase gene (adh) derived from Ectropis oblique, which has the nucleotide sequence shown in SEQ ID NO: 1. The invention also discloses a protein encoded by the gene adh, which has the amino acid sequence shown in SEQ ID NO: 2. The present invention further discloses the use of the gene adh in evaluating stress resistance and monitoring feeding environment of insects.

Description

technical field [0001] The invention belongs to the field of insect biotechnology. Specifically, the present invention relates to a cloning of the acetaldehyde dehydrogenase gene; it also relates to the evaluation of the resistance ability of insects such as tea geometrids and the monitoring of the growth environment of the insects by using the gene. Background technique [0002] In the process of studying properties such as insect biology, a large amount of insect material is required. Direct capture from nature is time-consuming and labor-intensive, and the work efficiency is poor. The artificial feeding method can rapidly expand the insect population, but unexplained deaths often occur during the insect breeding process, which brings losses to the insect breeding. [0003] Aldehydes are important components of peroxidation reaction products in organisms, such as malondialdehyde and acetaldehyde, which have strong toxic effects on organisms. Aldehyde dehydrogenase (adh)...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/53C12N9/04C12Q1/68
Inventor 林晨陆建良梁月荣郑新强董俊杰
Owner ZHEJIANG UNIV
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