Method of preparing corn polypeptide with rough material of zein

A technology of zein and corn polypeptide, which is applied in plant protein processing, etc., can solve problems such as complicated operation process, difficult to accurately grasp the pH of the process process, toxic and harmful substances, etc., and achieve simplified enzymatic hydrolysis production process, good quality and efficacy , the effect of high protein content

Inactive Publication Date: 2008-01-23
武汉天天好生物制品有限公司
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Problems solved by technology

However, the traditional alkali extraction and acid precipitation method is not only complicated, time-consuming and laborious to operate, but also difficult to accurately grasp the pH in the process, which will easily destroy the active ingredients in corn protein and generate toxic and harmful substances. Product quality is not easy to control
There are also technicians who choose protease to directly hydrolyze corn yellow powder to prepare corn peptides, but because corn yellow powder contains a large amount of starch and polysaccharides, the corn peptide products obtained by direct hydrolysis contain a large amount of polysaccharides or other impurities, resulting in corn peptide products The quality and efficacy of the

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  • Method of preparing corn polypeptide with rough material of zein

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] First, take zein powder with a total nitrogen content greater than or equal to 14% according to the ratio of material to liquid weight ratio of 1:15, put it into distilled water or pure water, stir evenly, and adjust its pH value with potassium hydroxide reagent. 7.5, and place the solution in a thermostat at 35° C. for 25 minutes, and keep stirring to fully dissolve the zein in it to obtain an aqueous solution of zein. Next, take the Alcalase alkaline protease whose enzyme activity unit is 200,000 U / g and whose weight accounts for 1% of the zein powder, and add it to the above-mentioned zein aqueous solution, and heat up to 60° C. During the enzymatic hydrolysis, stirring was continued, and the enzymatic hydrolysis time was maintained for 4 hours to obtain a zein enzymatic hydrolyzate. Then, the above-mentioned zein enzymatic hydrolysis solution was heated to boiling state, kept for 5 minutes to inactivate the enzyme activity, and when it was cooled to 80° C., coarse f...

Embodiment 2

[0021] First, take zein powder with a total nitrogen content greater than or equal to 14% according to the ratio of material to liquid weight ratio of 1:18, put it into distilled water or pure water, stir evenly, and adjust its pH value with potassium hydroxide reagent. 7.8, and place the solution in a thermostat at 40°C for 30 minutes, and keep stirring to fully dissolve the zein in it to obtain an aqueous solution of zein. Secondly, take the Alcalase alkaline protease whose enzyme activity unit is 200,000 U / g and whose weight accounts for 2% of zein powder, add it to the above-mentioned zein aqueous solution, and heat up to 55° C. During the enzymatic hydrolysis, stirring was continued, and the enzymatic hydrolysis time was maintained for 5 hours to obtain a zein enzymatic hydrolyzate. Then, the above-mentioned zein enzymatic hydrolysis solution was heated to boiling state, kept for 8 minutes to inactivate enzyme activity, and when it was cooled to 70° C., coarse filtration ...

Embodiment 3

[0023] First, take zein powder with a total nitrogen content greater than or equal to 14% according to the ratio of material to liquid weight ratio of 1:20, put it into distilled water or pure water, stir evenly, and adjust its pH value with potassium hydroxide reagent. 8.0, and the solution was kept in a thermostat at 45° C. for 35 minutes, and kept stirring to fully dissolve the zein in it to obtain an aqueous solution of zein. Secondly, take the Alcalase alkaline protease whose enzyme activity unit is 200,000 U / g and whose weight accounts for 3% of the zein powder, add it to the above-mentioned zein aqueous solution, and heat up to 50° C. During the enzymatic hydrolysis, stirring was continued, and the enzymatic hydrolysis time was maintained for 6 hours to obtain a zein enzymatic hydrolyzate. Then, the above-mentioned zein enzymatic hydrolysis solution was heated to boiling state, kept for 10 minutes to inactivate the enzyme activity, and when it was cooled to 75° C., coar...

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Abstract

The invention discloses a method for preparing corn polypeptide by taking zein as raw materials, which is designed for solving the defects that the traditional method of lifting alkali but sinking sour is easy to damage the active ingredient of the zein and the product has many impurities by hydrolyzing the yellow corn powder directly. The method is that: dissolving sufficiently the zein powder in alkaline water solution, conducting the limited zymohydrolysis on the zein with alkali protease and then undertaking the rough filter, decolor by using active carbon, bitter removing, fine filter, sterilization, vaccume compression, spraying and drying, thus achieving the corn polypeptide. The method selects the raw material with high content of proteide but without impurities as amylum, fibre and coloring matter, etc. and takes the alkaline water solution as the latent solvent which enables the zein dissolved sufficiently and facilitates the combination of the kali protease and the zein typsin and also enables the corn polypeptide product contain alkali ion which is good for human body. The corn polypeptide has the advantages of simple technics, easy controlled zymohydrolysis degree and good quality and effects.

Description

technical field [0001] The invention relates to the deep processing technology of corn food, in particular to a method for preparing corn polypeptide with zein as raw material. Background technique [0002] At present, most of the methods for preparing corn polypeptides use natural corn yellow powder as raw material. Firstly, the traditional alkali extraction and acid precipitation method is used to extract the zein in the corn yellow powder, and then the corn polypeptide is extracted by enzymatic hydrolysis of the zein. However, the traditional alkali extraction and acid precipitation method is not only complicated, time-consuming and laborious to operate, but also difficult to accurately grasp the pH in the process, which will easily destroy the active ingredients in corn protein and generate toxic and harmful substances. The quality of the product is not easy to control. There are also technicians who choose protease to directly hydrolyze corn yellow powder to prepare co...

Claims

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Application Information

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IPC IPC(8): A23J3/14
Inventor 陈栋梁莫朝晖吴冬梅
Owner 武汉天天好生物制品有限公司
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