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A siRNA capable of preventing and curing hair loss and accelerating hair growth, and preparation method and application thereof

A technology of SR-AR and pet-saak, applied in the application of siRNA in the prevention and treatment of hair loss and promotion of hair growth, the field of siRNA for prevention and treatment of hair loss and promotion of hair growth and its preparation, can solve the problems of difficult operation and promotion, high price and difficulty , to achieve the effect of improving stability and skin penetration

Inactive Publication Date: 2007-10-17
上海金璐生物科技有限公司 +2
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Problems solved by technology

However, the above methods are expensive, difficult to operate and difficult to promote

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  • A siRNA capable of preventing and curing hair loss and accelerating hair growth, and preparation method and application thereof
  • A siRNA capable of preventing and curing hair loss and accelerating hair growth, and preparation method and application thereof
  • A siRNA capable of preventing and curing hair loss and accelerating hair growth, and preparation method and application thereof

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Embodiment Construction

[0038] 1. Cloning of each expression element: According to the sequences of the human testosterone reductase and androgen receptor genes published by Genebank, the relevant fragment sequences were respectively selected, oligonucleotide fragments were designed and synthesized, and the oligonucleotide fragments were connected to obtain two The artificial gene fragment SRAR (for SEQ.ID.NO1) of the gene fragments, and the introduction of specific restriction endonuclease sites for cloning needs, according to the strategy shown in Figure 1 and Figure 2 to clone the double-stranded RNA expression vector pET -SRAR, the cloned expression plasmid was identified by PCR reaction and enzyme digestion.

[0039] 2. Fermentation production: Transform pET-SRAR into Escherichia coli [BL21-CodonPlus(DE3)-RIL] by conventional calcium chloride transformation method, pick a single colony and inoculate it into the LB medium containing 100 μg / mL ampicillin , cultivate overnight in a shaker at 37°C a...

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Abstract

The present invention belongs to biological preparation technology field, more specificly a siRNA for preventing hair loss and promoting hair growth and its preparation method and uses. It comprises: constructing long double-chain RNA(dsRNA) expression vector pET-SRAR related to preventing hair loss and promoting hair growth and expressing highly in E.Coli, transforming E.Coli and fermenting to produce long double-chain RNA, obtaining dsRNA, homogenizing for destroying cell wall and extracting E.Coli total RNA from fermented E.coli, purifying extract and obtaining dsRNA, hydrolyzing and purifying to obtain small interference RNA (siRNA),embedding siRNA using liposome, obtaining siRNA for preventing hair loss and promoting hair growth. The present invention has obvious effect on promoting hair growth.

Description

technical field [0001] The invention belongs to the technical field of biological preparations, and in particular relates to a siRNA for preventing and treating hair loss and promoting hair growth, a preparation method thereof, and the application of the siRNA in preventing and treating hair loss and promoting hair growth. technical background [0002] (1) About hair loss [0003] With the pollution of modern industrial production, changes in people's lifestyles and living standards, the pressure of daily work and the occurrence of genetic mutations, hair loss has become more and more common in the entire human society, and it has become more and more serious. It is difficult to achieve control of hair loss, resulting in various forms of baldness, which seriously affects people's physical and mental health and quality of life. The most common form of baldness is male pattern baldness, and every year, 60 million people in the United States (2 / 3 of men) spend about $1.5 billi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/11C12N15/70A61K31/7088A61P17/14C12N15/113
Inventor 王维荣黄伟达黄勇前谢承光
Owner 上海金璐生物科技有限公司
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