Listeria and toxicity fast test reagent kit

Abstract

A Lister bacterium and fast test reagent kit are disclosed. It consists of two freezing tubes containing different ingredients, 100 PCR reactive tubes and test specifications, the freezing tubes include TaqDNApolymerase, 10Xbuffer, dNTP, primer LM, LN, LS, LVSW, LGU, Ld, Lmo0038-1 and Lmo0038-2 and cracking buffer solution, positive control solution and diluting solution. The lowest testing limitreaches to 102FU / mL is correct, specific and sensitive.

Description

Listeria species and virulence rapid detection kit technical field The invention belongs to the field of biotechnology, and relates to the identification of pathogenic listeria in food, the diagnosis of animal listeriosis and the research on the classification and evolution of bacteria. Establish a rapid detection technology based on multiplex PCR to identify the various species of Listeria and distinguish the virulence of Listeria monocytogenes, that is, the rapid detection kit for Listeria species and virulence. Background technique Listeria (Listeria) bacteria are widely distributed and have strong tolerance to the environment. According to the genome sequence, virulence and ability to decompose sugar, etc., it can be divided into 6 species, namely Listeria monocytogenes (Listeria monocytogenes, Listeria monocytogenes for short), Listeria innocua , Listeria ivanovii, Listeria seligeri, Listeria welshimeri and Listeria grayi, of which Listeria monocytogenes and Listeria...

AI Technical Summary

Problems solved by technology

Listeria detection methods include trace biochemical reactions and serological methods, all of which have disadvantages such as time-consuming and labor-intensive operations, and large differences in test results.

At present, some molecular biological methods for the detection of Listeria spp. However, there is no report on a method that can simultaneously identify various species of Listeria and distinguish the virulence of Listeria monocytogenes

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