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Testosterone coma monad with aerobic denitrifying capability and method of treating waste water by the same

A Comamonas and aerobic denitrification technology, applied in the field of biological denitrification treatment of wastewater, can solve problems such as anoxic denitrification

Inactive Publication Date: 2007-08-15
BEIJING TECHNOLOGY AND BUSINESS UNIVERSITY
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AI Technical Summary

Problems solved by technology

This strain has the characteristics of denitrification under aerobic conditions. It can be inoculated in conventional aerobic nitrification sludge to realize simultaneous nitrification and denitrification of nitrogen-containing wastewater, which can solve the needs of biological denitrification in traditional wastewater treatment. The problem of adopting anoxic denitrification and aerobic nitrification in stages

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  • Testosterone coma monad with aerobic denitrifying capability and method of treating waste water by the same

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Effect test

Embodiment 1

[0014] Embodiment 1: Determination method of Comamonas testosteroni CGMCC No.1800 bacterial strain aerobic denitrification performance

[0015] In order to prevent the introduction of miscellaneous bacteria into the simulated wastewater, both the simulated nitrate wastewater I and the simulated nitrate wastewater II were inactivated in a high-temperature sterilization pot at 0.1 MPa for 20 minutes. Get 5ml of sterilized nitrate simulated waste water I and put it into a test tube, insert the pure Comamonas testosteroni CGMCC No.1800 bacterial strain sample preserved with liquid paraffin seal. The test tube was placed in an air bath constant temperature shaker, the constant temperature was maintained at 30°C, and the shaker speed was 160r / min to activate the strain. After activating for 12 hours, inoculate 10% of the inoculum into a 250ml Erlenmeyer flask filled with 200ml nitrate simulated waste water II to measure denitrification and denitrification performance. In order to e...

Embodiment 2-10

[0019] Embodiment 2-10: different experimental conditions, scope determine the best denitrification condition of Comamonas testosteroni CGMCC No.1800 bacterial strain

[0020] The present invention carries out 9 waste water treatment tests, preferably the aerobic denitrification reaction conditions of the Comamonas testosteroni WXZ-18 CGMCC No.1800 bacterial strain.

[0021] The four reaction factors of wastewater aerobic denitrification are: dissolved oxygen concentration, which is realized by controlling different rotational speeds rpm. The three levels of rotational speed are 60, 100 and 160 rpm, and the corresponding dissolved oxygen concentrations are 1.8-3.5 mg / L ( 24~46% saturated dissolved oxygen), 4.2~5.6mg / L (55~74% saturated dissolved oxygen) and 6.0~7.2mg / L (79~95% saturated dissolved oxygen); the three levels of nitro nitrogen concentration are respectively The three levels of carbon-nitrogen ratio are 2, 6 and 10 respectively; the three levels of pH are 6.5, 7.0 ...

Embodiment 11

[0026] Embodiment 11: PCR amplification and sequence determination of the 16SrRNA gene of Comamonas testosteroni CGMCC No.1800

[0027] Comamonas testosteroni CGMCC No.1800 was inoculated into 100 μL of double distilled water, boiled in a boiling water bath for 5 minutes, and colony PCR amplification was performed. The primers used for the 16SrRNA PCR reaction were a pair of universal primers, that is, the forward primer was f27 (5'-AGAGTTGATCCTGGCTCAG-3') and the reverse primer was r1492 (5'-GGTTACCTTGTTACGACTT-3'). Primers were synthesized by Beijing Huada Zhongsheng Technology Development Co., Ltd. PCR reaction system (50 μL): 5 μL of 10×Buffer, 4 μL of dNTPs, 1 μL of primers f27 and r1492, 39 μL of double distilled water, centrifuge and mix well, add 0.5 μL of DNA template, 0.5 μL of Taq enzyme. The PCR reaction program included: (1) pre-denaturation at 94°C for 5 min; (2) denaturation at 94°C for 1 min; (3) annealing at 50°C for 1 min; (4) extension at 72°C for 2 min for...

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Abstract

The invention discloses a primotest clustered hair single cell bacterium (Comamonas testosteroni) WXZ-18 CGMCC No.1800 strain with aerobic denitrifying property and a method to denitrify the waste water; which is characterized by the following: possessing strong tolerance degree for dissolved oxygen; deacidizing nitrate and nitrite with distinctive speed under the condition of 10-98% saturated dissolved oxygen; seeding to active sludge; proceeding enriched culture; realizing the aerobic anti-nitration.

Description

technical field [0001] The invention relates to a method for biological denitrification treatment of wastewater by using Comamonas testosteroni WXZ-18 CGMCC No.1800 bacterial strain under aerobic conditions. The strain has strong tolerance to dissolved oxygen, and can use nitrate and nitrite as electron acceptors under the condition of 10-98% saturated dissolved oxygen. Nitrate and nitrite in sewage can be reduced to gaseous products in an aerobic environment to denitrify wastewater. Background technique [0002] Among the various denitrification methods that have been applied so far, biological denitrification is still the main method in wastewater denitrification treatment. The traditional wastewater biological denitrification treatment process is: the organic nitrogen compounds in the sewage are first converted into ammonia nitrogen in the biochemical process, and the ammonia nitrogen is converted into nitrate and nitrite by autotrophic nitrifying bacteria in the nitrifi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C02F3/02C02F3/34
CPCY02W10/10
Inventor 汪苹翟茜项慕飞龙雯柯国华杨志
Owner BEIJING TECHNOLOGY AND BUSINESS UNIVERSITY
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