Process for treating straw by vapour explosive and alkaline oxydol oxydizing
A technology of oxidation coupling and steam explosion of straw, applied in biofuels, fermentation, etc., can solve the problems of low utilization rate of cellulase, achieve the effect of improving enzymatic hydrolysis conversion rate, increasing accessibility, and reducing dosage
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Embodiment 1
[0025] (1) 15g of wheat straw with a water content of 10% is put into a steam explosion tank and processed for 5 minutes at a steam pressure of 1.5Mpa, then dried at 60°C for 48 hours;
[0026] (2) Mix the dried steam-exploded wheat straw with the alkaline hydrogen peroxide solution in a ratio of 1:15 by weight, and oxidize it at 30°C for 12 hours; 1% NaOH and 1% H by weight 2 o 2 mixed solution;
[0027] (3) Filter the mixed system after the reaction in step (2), wash the obtained residue with water until neutral, and dry at 60°C (dry for 48 hours);
[0028] (4) The dry residue (steam-exploded wheat straw) was enzymolyzed for 48 hours with the cellulase hydrolyzate prepared by pH4.8 buffer solution at 40°C, the amount of cellulase was 10IU / g substrate, and the solid-to-liquid ratio was 1:5. The sugar concentration of the obtained enzymatic hydrolyzate was 104.68g / l.
Embodiment 2
[0030] (1) 15 g of corn stalks with a water content of 35% were put into a steam explosion tank and treated at a steam pressure of 1.0 Mpa for 8 minutes, and then dried at 60° C. for 48 hours;
[0031] (2) Mix the dried steam-exploded corn stalks with the alkaline hydrogen peroxide solution at a ratio of 1:20 by weight, and oxidize it at 40°C for 24 hours; the alkaline hydrogen peroxide solution is a The concentration is 0.5% KOH and the weight percent concentration is 10% H 2 o 2 mixed solution;
[0032] (3) Filter the mixed system after the reaction in step 2, wash the obtained residue with water until neutral, and dry at 60°C (dry for 48 hours);
[0033] (4) Enzymolyze the dried residue (steam-exploded corn stalk) at 50°C with pH 4.8 buffer solution for 48 hours, the dosage of cellulase is 15IU / g substrate, the solid-liquid ratio It is 1:65. The sugar concentration of the obtained enzymatic hydrolyzate was 109.42g / l.
Embodiment 3
[0035] (1) 15g of sorghum stalks with a water content of 15% are put into a steam explosion tank and processed for 10 minutes at a steam pressure of 1.5Mpa, and then dried at 60°C for 48 hours;
[0036] (2) Mix the dried steam-exploded sorghum stalks with the alkaline hydrogen peroxide solution in a ratio of 1:20 by weight, and oxidize it at 30°C for 36 hours; the alkaline hydrogen peroxide solution is a The concentration is 1% Ca(OH) 2 and a weight percent concentration of 12% H 2 o 2 mixed solution;
[0037] (3) Filter the mixed system after the reaction in step 2, wash the obtained residue with water until neutral, and dry at 60°C (dry for 48 hours);
[0038] (4) Enzymolyze the dried residue (steam-exploded sorghum stalk) at 45°C with a pH4.8 buffer solution for 72 hours. The dosage of cellulase is 20IU / g substrate, and the solid-liquid ratio It is 1:5. The sugar concentration of the obtained enzymatic hydrolyzate was 110.97g / l.
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